Cloned (Comment) | Organism |
---|---|
overexpression of C73F, C289F, C73S, C289S and C73/C289S mutant enzyme in Escherichia coli | Geobacillus stearothermophilus |
Protein Variants | Comment | Organism |
---|---|---|
C289F | approx. 1/13 of wild-type activity at 55°C | Geobacillus stearothermophilus |
C289S | similar activity like wild-type | Geobacillus stearothermophilus |
C73F | heat sensitive mutant | Geobacillus stearothermophilus |
C73S | similar activity like wild-type | Geobacillus stearothermophilus |
C73S/C289S | similar activity like wild-type | Geobacillus stearothermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
p-chloromercuribenzoic acid | 0.15 mM, 882% inhibition of wild-type farnesyl diphosphate synthase, 11% inhibition of C73S/C289S mutant enzyme | Geobacillus stearothermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.7 | - |
isopentenyl diphosphate | wild-type enzyme at 45°C | Geobacillus stearothermophilus | |
4.8 | - |
isopentenyl diphosphate | C73F mutant enzyme at 45°C | Geobacillus stearothermophilus | |
4.9 | - |
geranyl diphosphate | C73F mutant enzyme at 45°C | Geobacillus stearothermophilus | |
5.6 | - |
geranyl diphosphate | C73S/C289S mutant enzyme at 55°C | Geobacillus stearothermophilus | |
5.8 | - |
geranyl diphosphate | wild-type enzyme at 45°C | Geobacillus stearothermophilus | |
6.4 | - |
geranyl diphosphate | C289S mutant enzyme at 55°C | Geobacillus stearothermophilus | |
7.3 | - |
geranyl diphosphate | C73F mutant enzyme at 55°C | Geobacillus stearothermophilus | |
7.5 | - |
geranyl diphosphate | C289F mutant enzyme at 55°C | Geobacillus stearothermophilus | |
8.4 | - |
geranyl diphosphate | wild-type enzyme at 55°C | Geobacillus stearothermophilus | |
11.6 | - |
isopentenyl diphosphate | C73F mutant enzyme at 55°C | Geobacillus stearothermophilus | |
13 | - |
isopentenyl diphosphate | wild-type enzyme at 55°C | Geobacillus stearothermophilus | |
13.3 | - |
isopentenyl diphosphate | C73S/C289S mutant enzyme at 55°C | Geobacillus stearothermophilus | |
14.3 | - |
isopentenyl diphosphate | C289S mutant enzyme at 55°C | Geobacillus stearothermophilus | |
130 | - |
isopentenyl diphosphate | C289F mutant enzyme at 55°C | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Oxidation Stability | Organism |
---|---|
enzyme seems to be susceptible to oxidation when treated with Cu2+, forming an intrasubunit disulfide bond rather than intersubunit bonds | Geobacillus stearothermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.35 | - |
C289F mutant enzyme at 55°C | Geobacillus stearothermophilus |
1.3 | - |
C73F mutant enzyme at 45°C | Geobacillus stearothermophilus |
2.61 | - |
wild-type enzyme at 45°C | Geobacillus stearothermophilus |
3.94 | - |
C289S mutant enzyme at 55°C | Geobacillus stearothermophilus |
3.97 | - |
C73S/C289S mutant enzyme at 55°C | Geobacillus stearothermophilus |
4.3 | - |
C73F mutant enzyme at 55°C | Geobacillus stearothermophilus |
4.69 | - |
wild-type enzyme at 55°C | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + 2 isopentenyl diphosphate | - |
Geobacillus stearothermophilus | (E,E)-farnesyl diphosphate + 2 diphosphate | - |
? | |
geranyl diphosphate + isopentenyl diphosphate | - |
Geobacillus stearothermophilus | diphosphate + farnesyl diphosphate | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
wild-type: stable for 30 min, C73F mutant: complete loss of activity after 20 min | Geobacillus stearothermophilus |