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Literature summary for 2.5.1.100 extracted from
- Reverse prenyltransferase in the biosynthesis of fumigaclavine C in Aspergillus fumigatus: gene expression, purification, and characterization of fumigaclavine C synthase FGAPT1 (2006), ChemBioChem, 7, 158-164.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene fgaPT1, DNA and amino acid sequence determination and analysis, overexpression of the His6-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS | Aspergillus fumigatus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | 90% inhibition at 5 mM | Aspergillus fumigatus |  |
| Zn2+ | 90% inhibition at 5 mM | Aspergillus fumigatus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.006 | - |
fumigaclavine A | pH 7.5, 30°C | Aspergillus fumigatus | |
| 0.013 | - |
dimethylallyl diphosphate | pH 7.5, 30°C | Aspergillus fumigatus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Aspergillus fumigatus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no significant effects by Mg2+, Ca2+, and EDTA at 5 mM | Aspergillus fumigatus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 110000 | - |
recombinant His6-tagged FgaPT1, gel filtration | Aspergillus fumigatus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| fumigaclavine A + dimethylallyl diphosphate | Aspergillus fumigatus | - |
fumigaclavine C + diphosphate | - |
? | |
| fumigaclavine A + dimethylallyl diphosphate | Aspergillus fumigatus Af293 | - |
fumigaclavine C + diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus fumigatus | - |
gene fgaPT1 | - |
| Aspergillus fumigatus Af293 | - |
gene fgaPT1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged FgaPT1 from Escherichia coli strain BL21(DE3)pLysS to near homogeneity | Aspergillus fumigatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| fumigaclavine A + dimethylallyl diphosphate | - |
Aspergillus fumigatus | fumigaclavine C + diphosphate | - |
? | |
| fumigaclavine A + dimethylallyl diphosphate | the enzyme converts fumigaclavine A into fumigaclavine C by attaching a dimethylallyl moiety to C-2 of the indole nucleus in a reverse manner, that is, by connection of C-3 of the dimethylallyl moiety to an aromatic nucleus | Aspergillus fumigatus | fumigaclavine C + diphosphate | - |
? | |
| fumigaclavine A + dimethylallyl diphosphate | - |
Aspergillus fumigatus Af293 | fumigaclavine C + diphosphate | - |
? | |
| fumigaclavine A + dimethylallyl diphosphate | the enzyme converts fumigaclavine A into fumigaclavine C by attaching a dimethylallyl moiety to C-2 of the indole nucleus in a reverse manner, that is, by connection of C-3 of the dimethylallyl moiety to an aromatic nucleus | Aspergillus fumigatus Af293 | fumigaclavine C + diphosphate | - |
? | |
| additional information | FgaPT1 shows relatively strict substrate specificity towards fumigaclavine A | Aspergillus fumigatus | ? | - |
? | |
| additional information | FgaPT1 shows relatively strict substrate specificity towards fumigaclavine A | Aspergillus fumigatus Af293 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 x 53000, recombinant His6-tagged FgaPT1, SDS-PAGE | Aspergillus fumigatus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FgaPT1 | - |
Aspergillus fumigatus |
| fumigaclavine C synthase | - |
Aspergillus fumigatus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Aspergillus fumigatus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.8 | - |
dimethylallyl diphosphate | pH 7.5, 30°C | Aspergillus fumigatus | |
| 0.8 | - |
fumigaclavine A | pH 7.5, 30°C | Aspergillus fumigatus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Aspergillus fumigatus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | FgaPT1 shows very low sequence similarity to the recently identified prenyltransferase LtxC from cyanobacteria, which also catalyzes the reverse prenylation of an indole nucleus | Aspergillus fumigatus |
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