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Literature summary for 2.5.1.101 extracted from

  • Knirel, Y.; Senchenkova, S.; Kocharova, N.; Shashkov, A.; Helbig, J.; Zaehringer, U.
    Identification of a homopolymer of 5-acetamidino-7-acetamido-3,5,7,9-tetradeoxy-D-glycero-D-talo-nonulosonic acid in the lipopolysaccharides of Legionella pneumophila non-1 serogroups (2001), Biochemistry, 66, 1035-1041.
    View publication on PubMed
Search for more literature for 2.5.1.101

Cloned(Commentary)

Cloned (Comment) Organism
gene lpg 0752, cloning and expression of NeuB results in insoluble protein which possesses very low levels of N,N'-diacetyllegionaminate synthase activity Legionella pneumophila

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2,4-diacetamido-2,4,6-trideoxy-alpha-D-mannopyranose + phosphoenolpyruvate + H2O Legionella pneumophila
-
 N,N'-diacetyllegionaminate + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Legionella pneumophila
-
 serogroups 2-14, and strains Lansing 3 and 16453-92 from another serogroup, gene lpg 0752 or neuB homologue
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4-diacetamido-2,4,6-trideoxy-alpha-D-mannopyranose + phosphoenolpyruvate + H2O
-
 Legionella pneumophila N,N'-diacetyllegionaminate + phosphate
-
 ?
2,4-diacetamido-2,4,6-trideoxy-alpha-D-mannopyranose + phosphoenolpyruvate + H2O
-
 Legionella pneumophila N,N'-diacetyllegionaminate + phosphate i.e. Leg5Ac7Ac ?

Synonyms

Synonyms Comment Organism
NeuB
-
 Legionella pneumophila
nonulosonic acid synthase
-
 Legionella pneumophila

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Legionella pneumophila

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Legionella pneumophila

General Information

General Information Comment Organism
metabolism the enzyme is involved in the the Leg pathway, which is essential for the biosynthesis of CMP-N,N'-diacetyllegionaminate, that acts as a donor substrate with a selection of bacterial and mammalian sialyltransferases Legionella pneumophila
additional information NeuB can be substituted by NanA from Escherichia coli, a sialic acid aldolase that catalyzes the condensation of 2,4-diacetamido-2,4,6-trideoxy-alpha-D-mannopyranose with pyruvate resulting in N,N'-diacetyllegionaminate formation, ability of sialyltransferases to utilize the CMP-N,N'-diacetyllegionaminate donor, overview Legionella pneumophila