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Literature summary for 2.5.1.108 extracted from

  • Dong, M.; Zhang, Y.; Lin, H.
    Methods for studying the radical SAM enzymes in diphthamide biosynthesis (2018), Methods Enzymol., 606, 421-438 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.5.1.108

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Saccharomyces cerevisiae
expressed in Escherichia coli BL21(DE3) cells Pyrococcus horikoshii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + L-histidine-[translation elongation factor 2] Saccharomyces cerevisiae
-
 S-methyl-5'-thioadenosine + 2-[(3S)-3-amino-3-carboxypropyl]-L-histidine-[translation elongation factor 2]
-
 ?
S-adenosyl-L-methionine + L-histidine-[translation elongation factor 2] Pyrococcus horikoshii
-
 S-methyl-5'-thioadenosine + 2-[(3S)-3-amino-3-carboxypropyl]-L-histidine-[translation elongation factor 2]
-
 ?
S-adenosyl-L-methionine + L-histidine-[translation elongation factor 2] Saccharomyces cerevisiae BY4741
-
 S-methyl-5'-thioadenosine + 2-[(3S)-3-amino-3-carboxypropyl]-L-histidine-[translation elongation factor 2]
-
 ?

Organism

Organism UniProt Comment Textmining
Pyrococcus horikoshii
-
-
-
Saccharomyces cerevisiae
-
-
-
Saccharomyces cerevisiae BY4741
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose resin column chromatography Saccharomyces cerevisiae
Ni-NTA agarose resin column chromatography Pyrococcus horikoshii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + L-histidine-[translation elongation factor 2]
-
 Saccharomyces cerevisiae S-methyl-5'-thioadenosine + 2-[(3S)-3-amino-3-carboxypropyl]-L-histidine-[translation elongation factor 2]
-
 ?
S-adenosyl-L-methionine + L-histidine-[translation elongation factor 2]
-
 Pyrococcus horikoshii S-methyl-5'-thioadenosine + 2-[(3S)-3-amino-3-carboxypropyl]-L-histidine-[translation elongation factor 2]
-
 ?
S-adenosyl-L-methionine + L-histidine-[translation elongation factor 2]
-
 Saccharomyces cerevisiae BY4741 S-methyl-5'-thioadenosine + 2-[(3S)-3-amino-3-carboxypropyl]-L-histidine-[translation elongation factor 2]
-
 ?

Subunits

Subunits Comment Organism
heterodimer 1 * 60000 + 1 * 40000, SDS-PAGE Saccharomyces cerevisiae
homodimer 2 * 40000, SDS-PAGE Pyrococcus horikoshii

Synonyms

Synonyms Comment Organism
diphthamide biosynthesis protein 2
-
 Pyrococcus horikoshii
Dph1-Dph2
-
 Saccharomyces cerevisiae
Dph2
-
 Pyrococcus horikoshii

Cofactor

Cofactor Comment Organism Structure
[4Fe-4S]-center
-
 Saccharomyces cerevisiae
[4Fe-4S]-center
-
 Pyrococcus horikoshii