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Literature summary for 2.5.1.110 extracted from
- FtmPT2, an N-prenyltransferase from Aspergillus fumigatus, catalyses the last step in the biosynthesis of fumitremorgin B (2008), ChemBioChem, 9, 2059-2063.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Saccharomyces cerevisiae | Aspergillus fumigatus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.096 | - |
12alpha,13alpha-dihydroxyfumitremorgin C | 37°C, pH not specified in the publication | Aspergillus fumigatus |  |
| 0.186 | - |
dimethylallyl diphosphate | 37°C, pH not specified in the publication | Aspergillus fumigatus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | product formation is independent of the presence of metal ions. Even in the presence of EDTA no loss of enzyme activity is not detected | Aspergillus fumigatus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50100 | - |
2 * 50100, SDS-PAGE | Aspergillus fumigatus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dimethylallyl diphosphate + 12alpha,13alpha-dihydroxyfumitremorgin C | Aspergillus fumigatus | the enzyme is involved in the biosynthetic pathways of several indole alkaloids such as fumitremorgins and verruculogen | diphosphate + fumitremorgin B | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus fumigatus | B9WZX7 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aspergillus fumigatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dimethylallyl diphosphate + 12alpha,13alpha-dihydroxyfumitremorgin C | the enzyme is involved in the biosynthetic pathways of several indole alkaloids such as fumitremorgins and verruculogen | Aspergillus fumigatus | diphosphate + fumitremorgin B | - |
? | |
| dimethylallyl diphosphate + 12alpha,13alpha-dihydroxyfumitremorgin C | the enzyme is specific for dimethylallyl diphosphate as prenyl donor, no activity with geranyl diphosphate. Relatively high specificity towards the aromatic substrate, it is extremely regiospecific | Aspergillus fumigatus | diphosphate + fumitremorgin B | - |
? | |
| dimethylallyl diphosphate + fumitremorgin C | relative activity of 10% compared to 12,13-dihydroxyfumitremorgin C | Aspergillus fumigatus | ? | - |
? | |
| additional information | no activity with brevianamide F, tryprostatin A or B | Aspergillus fumigatus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 50100, SDS-PAGE | Aspergillus fumigatus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ftmH | - |
Aspergillus fumigatus |
| FtmPT2 | - |
Aspergillus fumigatus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Aspergillus fumigatus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03 | - |
dimethylallyl diphosphate | 37°C, pH not specified in the publication | Aspergillus fumigatus | |
| 0.03 | - |
12alpha,13alpha-dihydroxyfumitremorgin C | 37°C, pH not specified in the publication | Aspergillus fumigatus | |
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