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Literature summary for 2.5.1.112 extracted from

  • Brugiere, N.; Humbert, S.; Rizzo, N.; Bohn, J.; Habben, J.E.
    A member of the maize isopentenyl transferase gene family, Zea mays isopentenyl transferase 2 (ZmIPT2), encodes a cytokinin biosynthetic enzyme expressed during kernel development. Cytokinin biosynthesis in maize (2008), Plant Mol. Biol., 67, 215-229.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-Star cells Zea mays

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dimethylallyl diphosphate + ADP Zea mays
-
diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
-
?
dimethylallyl diphosphate + AMP Zea mays low efficiency diphosphate + N6-(dimethylallyl)adenosine phosphate
-
?
dimethylallyl diphosphate + ATP Zea mays
-
diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
-
?
additional information Zea mays 1-hydroxy-2-methyl-2-buten-4-yl 4-diphosphate is not a substrate ?
-
?

Organism

Organism UniProt Comment Textmining
Zea mays
-
cultivar B73
-

Purification (Commentary)

Purification (Comment) Organism
Talon metal affinity column chromatography Zea mays

Source Tissue

Source Tissue Comment Organism Textmining
embryo
-
Zea mays
-
endosperm
-
Zea mays
-
kernel strong expression in developing kernel Zea mays
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dimethylallyl diphosphate + ADP
-
Zea mays diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
-
?
dimethylallyl diphosphate + AMP low efficiency Zea mays diphosphate + N6-(dimethylallyl)adenosine phosphate
-
?
dimethylallyl diphosphate + ATP
-
Zea mays diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
-
?
additional information 1-hydroxy-2-methyl-2-buten-4-yl 4-diphosphate is not a substrate Zea mays ?
-
?

Synonyms

Synonyms Comment Organism
IPT2
-
Zea mays
isopentenyl transferase 2
-
Zea mays

Cofactor

Cofactor Comment Organism Structure
ADP the enzyme preferentially uses ADP and ATP over AMP Zea mays
ATP the enzyme preferentially uses ADP and ATP over AMP Zea mays

Expression

Organism Comment Expression
Zea mays enzyme expression is developmentally regulated in kernel tissues and coincides with cytokinin accumulation. The expression gradually increases from 6 to 8 days after pollination (DAP), peaks at 8 DAP, and decreases gradually until 14 DAP before increasing again at later stages (15-34 DAP) up

General Information

General Information Comment Organism
physiological function the enzyme is expressed during kernel development Zea mays