Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21-Star cells | Zea mays |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + ADP | Zea mays | - |
diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate | - |
? | |
dimethylallyl diphosphate + AMP | Zea mays | low efficiency | diphosphate + N6-(dimethylallyl)adenosine phosphate | - |
? | |
dimethylallyl diphosphate + ATP | Zea mays | - |
diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate | - |
? | |
additional information | Zea mays | 1-hydroxy-2-methyl-2-buten-4-yl 4-diphosphate is not a substrate | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | - |
cultivar B73 | - |
Purification (Comment) | Organism |
---|---|
Talon metal affinity column chromatography | Zea mays |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | - |
Zea mays | - |
endosperm | - |
Zea mays | - |
kernel | strong expression in developing kernel | Zea mays | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + ADP | - |
Zea mays | diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate | - |
? | |
dimethylallyl diphosphate + AMP | low efficiency | Zea mays | diphosphate + N6-(dimethylallyl)adenosine phosphate | - |
? | |
dimethylallyl diphosphate + ATP | - |
Zea mays | diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate | - |
? | |
additional information | 1-hydroxy-2-methyl-2-buten-4-yl 4-diphosphate is not a substrate | Zea mays | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
IPT2 | - |
Zea mays |
isopentenyl transferase 2 | - |
Zea mays |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ADP | the enzyme preferentially uses ADP and ATP over AMP | Zea mays | |
ATP | the enzyme preferentially uses ADP and ATP over AMP | Zea mays |
Organism | Comment | Expression |
---|---|---|
Zea mays | enzyme expression is developmentally regulated in kernel tissues and coincides with cytokinin accumulation. The expression gradually increases from 6 to 8 days after pollination (DAP), peaks at 8 DAP, and decreases gradually until 14 DAP before increasing again at later stages (15-34 DAP) | up |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is expressed during kernel development | Zea mays |