Application | Comment | Organism |
---|---|---|
synthesis | the specific 4-O-prenylation of tyrosine derivatives by SirD could find usage for production of prenylated tyrosine derivatives by chemoenzymatic synthesis | Leptosphaeria maculans |
Cloned (Comment) | Organism |
---|---|
overproduction of His6-SirD in Escherichia coli | Leptosphaeria maculans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + L-tyrosine | Leptosphaeria maculans | the enzyme is involved in biosynthesis of sirodesmin PL | diphosphate + 4-O-dimethylallyl-L-tyrosine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leptosphaeria maculans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Leptosphaeria maculans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + 3-amino-L-tyrosine | SirD catalyzes the regiospecific O-prenylation at position C4 of tyrosine and derivatives. 3-Amino-L-tyrosine is prenylated with 108% of the activity compared to L-tyrosine | Leptosphaeria maculans | diphosphate + 3-amino-4-O-dimethylallyl-L-tyrosine | - |
? | |
dimethylallyl diphosphate + 3-fluoro-DL-tyrosine | SirD catalyzes the regiospecific O-prenylation at position C4 of tyrosine and derivatives. 3-Iodo-L-tyrosine is prenylated with 53.6% of the activity compared to L-tyrosine | Leptosphaeria maculans | diphosphate + 4-O-dimethylallyl-3-fluoro-DL-tyrosine | - |
? | |
dimethylallyl diphosphate + 3-iodo-L-tyrosine | SirD catalyzes the regiospecific O-prenylation at position C4 of tyrosine and derivatives. 3-Iodo-L-tyrosine is prenylated with 27.3% of the activity compared to L-tyrosine | Leptosphaeria maculans | diphosphate + 4-O-dimethylallyl-3-iodo-L-tyrosine | - |
? | |
dimethylallyl diphosphate + L-tyrosine | the enzyme is involved in biosynthesis of sirodesmin PL | Leptosphaeria maculans | diphosphate + 4-O-dimethylallyl-L-tyrosine | - |
? | |
dimethylallyl diphosphate + L-tyrosine | SirD catalyzes the regiospecific O-prenylation at position C4 of tyrosine and derivatives | Leptosphaeria maculans | diphosphate + 4-O-dimethylallyl-L-tyrosine | - |
? | |
additional information | SirD also catalyses the C-prenylation of L-Trp derivatives, in addition to the O-prenylation | Leptosphaeria maculans | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SirD | - |
Leptosphaeria maculans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Leptosphaeria maculans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Leptosphaeria maculans |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in biosynthesis of sirodesmin PL | Leptosphaeria maculans |