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Literature summary for 2.5.1.128 extracted from

  • Okada, K.; Hidese, R.; Fukuda, W.; Niitsu, M.; Takao, K.; Horai, Y.; Umezawa, N.; Higuchi, T.; Oshima, T.; Yoshikawa, Y.; Imanaka, T.; Fujiwara, S.
    Identification of a novel aminopropyltransferase involved in the synthesis of branched-chain polyamines in hyperthermophiles (2014), J. Bacteriol., 196, 1866-1876.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermococcus kodakarensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 S-adenosyl 3-(methylthio)propylamine + spermidine Thermococcus kodakarensis N4-bis(aminopropyl)spermidine is the major polyamine in Thermococcus kodakarensis. The branched-chain polyamine synthesized by BpsA is important for cell growth at 93°C 2 S-methyl-5'-thioadenosine + N4-bis(aminopropyl)spermidine overall reaction ?

Organism

Organism UniProt Comment Textmining
Thermococcus kodakarensis Q5JIZ3
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Thermococcus kodakarensis

Reaction

Reaction Comment Organism Reaction ID
2 S-adenosyl 3-(methylsulfanyl)propylamine + spermidine = 2 S-methyl-5'-thioadenosine + N4-bis(aminopropyl)spermidine overall reaction Thermococcus kodakarensis
S-adenosyl 3-(methylsulfanyl)propylamine + N4-aminopropylspermidine = S-methyl-5'-thioadenosine + N4-bis(aminopropyl)spermidine (1b) Thermococcus kodakarensis
S-adenosyl 3-(methylsulfanyl)propylamine + spermidine = S-methyl-5'-thioadenosine + N4-aminopropylspermidine (1a) Thermococcus kodakarensis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.12
-
pH 9.0, 70°C, production of N4-aminopropylspermine Thermococcus kodakarensis
0.34
-
pH 9.0, 70°C, production of N4-bis(aminopropyl)spermidine Thermococcus kodakarensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 S-adenosyl 3-(methylthio)propylamine + spermidine
-
Thermococcus kodakarensis 2 S-methyl-5'-thioadenosine + N4-bis(aminopropyl)spermidine overall reaction. N4-bis(aminopropyl)spermidine is produced from spermidine by the sequential reactions catalyzed by BpsA via N4-aminopropylspermidine ?
2 S-adenosyl 3-(methylthio)propylamine + spermidine N4-bis(aminopropyl)spermidine is the major polyamine in Thermococcus kodakarensis. The branched-chain polyamine synthesized by BpsA is important for cell growth at 93°C Thermococcus kodakarensis 2 S-methyl-5'-thioadenosine + N4-bis(aminopropyl)spermidine overall reaction ?
S-adenosyl 3-(methylthio)propylamine + N4-aminopropylspermine
-
Thermococcus kodakarensis S-methyl-5'-thioadenosine + N4-bis(aminopropyl)spermidine
-
?
S-adenosyl 3-(methylthio)propylamine + spermidine
-
Thermococcus kodakarensis S-methyl-5'-thioadenosine + N4-aminopropylspermidine
-
?
S-adenosyl 3-(methylthio)propylamine + spermine
-
Thermococcus kodakarensis S-methyl-5'-thioadenosine + N4-aminopropylspermine
-
?

Synonyms

Synonyms Comment Organism
BpsA
-
Thermococcus kodakarensis
TK1691 locus name Thermococcus kodakarensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
assay at Thermococcus kodakarensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
assay at Thermococcus kodakarensis

General Information

General Information Comment Organism
malfunction at 85°C the bspA disruptant strain shows a slightly extended lag phase compared to that of the wild-type strain. At 93°C there is no cell growth of the disruptant strain. The bpsA gene is required for growth at the higher temperature. The growth defect of the disruptant strain at 93°C is partially restored by the addition of 1 mM N4-bis(aminopropyl)spermidine to the medium. N4-bis(Aminopropyl)spermidine is required for cell growth of Thermococcus kodakarensis in a higher-temperature environment Thermococcus kodakarensis
physiological function N4-bis(aminopropyl)spermidine is the major polyamine in Thermococcus kodakarensis. The branched-chain polyamine synthesized by BpsA is important for cell growth at 93°C Thermococcus kodakarensis