Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | the enzyme is active in the absence of exogenously added divalent cations but lost all activity when incubated before assay with 5 mM EDTA. The lost enzyme activity is restored to 2.43 times higher levels of the original one by the addition of 1 mM MnCl2, CoCl2, or NiCl2 and to one-third the original activity by 1 mM MgCl2. The other cations (Ca2+, Fe2+, Cu2+, and Zn2+) do not restore enzyme activity | Oncorhynchus mykiss | |
NaCl | activity is reduced by about 50% in the presence of 150 mM NaCl compared with the activity without added NaCl | Oncorhynchus mykiss |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.66 | - |
D-mannose 6-phosphate | pH 7.0, 25°C | Oncorhynchus mykiss |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | maximal activity in presence of Mn2+ | Oncorhynchus mykiss |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
gel filtration | Oncorhynchus mykiss |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + D-mannose 6-phosphate + H2O | Oncorhynchus mykiss | the enzyme participates in the biosynthesis of the sialic acid 3-deoxy-D-glycero-D-galacto-nononate | 3-deoxy-D-glycero-D-galacto-nononate 9-phosphate + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oncorhynchus mykiss | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Oncorhynchus mykiss |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | specific activity is 33fold lower than in testis | Oncorhynchus mykiss | - |
testis | - |
Oncorhynchus mykiss | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0009 | - |
pH 7.0, 25°C, the 50fold purified enzyme from testis is unstable, preventing further purification of this enzyme activity | Oncorhynchus mykiss |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + D-mannose 6-phosphate + H2O | the enzyme participates in the biosynthesis of the sialic acid 3-deoxy-D-glycero-D-galacto-nononate | Oncorhynchus mykiss | 3-deoxy-D-glycero-D-galacto-nononate 9-phosphate + phosphate | - |
? | |
phosphoenolpyruvate + D-mannose 6-phosphate + H2O | phosphoenolpyruvate i.e. 2-(phosphonooxy)-2-propenoate | Oncorhynchus mykiss | 3-deoxy-D-glycero-D-galacto-nononate 9-phosphate + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
2-keto-3-deoxy-D-glycero-D-galacto-9-phosphonononic acid synthase | - |
Oncorhynchus mykiss |
KDN-9-phosphate synthetase | - |
Oncorhynchus mykiss |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Oncorhynchus mykiss |