Cloned (Comment) | Organism |
---|---|
expressed under the control of the T5 promoter in a DHPS-deficient Escherichia coli strain | Plasmodium falciparum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dapsone | - |
Plasmodium falciparum | |
Guanidine HCl | 0.25 M, 50% inhibition | Plasmodium falciparum | |
KCl | above 0.4 M | Plasmodium falciparum | |
NaCl | above 0.2 M | Plasmodium falciparum | |
p-Aminosalicylic acid | - |
Plasmodium falciparum | |
sulfacetamide | - |
Plasmodium falciparum | |
sulfachloropyridazine | - |
Plasmodium falciparum | |
Sulfadiazine | - |
Plasmodium falciparum | |
sulfadimethoxine | - |
Plasmodium falciparum | |
sulfadoxine | - |
Plasmodium falciparum | |
sulfamerazine | - |
Plasmodium falciparum | |
sulfamethoxazole | - |
Plasmodium falciparum | |
sulfamethoxypyridazine | - |
Plasmodium falciparum | |
sulfamoxole | - |
Plasmodium falciparum | |
sulfanilamide | - |
Plasmodium falciparum | |
sulfapyridine | - |
Plasmodium falciparum | |
sulfaquinoxaline | - |
Plasmodium falciparum | |
sulfathiazole | - |
Plasmodium falciparum | |
sulfisoxazole | - |
Plasmodium falciparum | |
Urea | 0.9 M, 50% inhibition | Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00122 | - |
2-Amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine | - |
Plasmodium falciparum | |
0.00125 | - |
4-Aminobenzoate | - |
Plasmodium falciparum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | 0.4 M activates | Plasmodium falciparum | |
Mg2+ | dependent on | Plasmodium falciparum | |
NaCl | 0.2 M activates | Plasmodium falciparum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
83000 | - |
bifunctional hydroxymethyldihydropterin pyrophosphokinase-dihydropteroate synthase, gel filtration | Plasmodium falciparum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant hydroxymethyldihydropterin pyrophosphokinase-dihydropteroate synthase | Plasmodium falciparum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0106 | - |
- |
Plasmodium falciparum |
Storage Stability | Organism |
---|---|
-20°C and -80°C stable for 3 months in 20 mM TrisHCl, pH 8.0 buffer containing 10% or 20% glycerol | Plasmodium falciparum |
-20°C or -80°C, in buffer without glycerol, 30% and 50% of the enzyme activity is lost when stored for 6 months | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine + 4-aminobenzoate | - |
Plasmodium falciparum | 7,8-dihydropteroate + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | - |
Plasmodium falciparum |
Synonyms | Comment | Organism |
---|---|---|
hydroxymethyldihydropterin pyrophosphokinase-dihydropteroate synthase | bifunctional enzyme | Plasmodium falciparum |
pfPPK-DHPS | recombinant hydroxymethyldihydropterin pyrophosphokinase-dihydropteroate synthase | Plasmodium falciparum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 45 | - |
Plasmodium falciparum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 55 | 30°C: about 40% of maximal activity, 55°C: about 10% of maximal activity | Plasmodium falciparum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
inactivation above | Plasmodium falciparum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
- |
Plasmodium falciparum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8.5 | 10.5 | pH 8.5: about 40% of maximal activity, pH 10.5: about 85% of maximal activity | Plasmodium falciparum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0006 | - |
sulfathiazole | - |
Plasmodium falciparum | |
0.001 | - |
dapsone | - |
Plasmodium falciparum | |
0.0015 | - |
sulfachloropyridazine | - |
Plasmodium falciparum | |
0.0016 | - |
sulfaquinoxaline | - |
Plasmodium falciparum | |
0.0018 | - |
sulfadimethoxine | - |
Plasmodium falciparum | |
0.0019 | - |
sulfapyridine | - |
Plasmodium falciparum | |
0.0027 | - |
sulfamoxole | - |
Plasmodium falciparum | |
0.0033 | - |
sulfamethoxypyridazine | - |
Plasmodium falciparum | |
0.0044 | - |
sulfamethoxazole | - |
Plasmodium falciparum | |
0.0048 | - |
sulfamerazine | - |
Plasmodium falciparum | |
0.0068 | - |
Sulfadiazine | - |
Plasmodium falciparum | |
0.0076 | - |
sulfisoxazole | - |
Plasmodium falciparum | |
0.0082 | - |
p-Aminosalicylic acid | - |
Plasmodium falciparum | |
0.011 | - |
sulfadoxine | - |
Plasmodium falciparum | |
0.0437 | - |
sulfanilamide | - |
Plasmodium falciparum | |
0.0941 | - |
sulfacetamide | - |
Plasmodium falciparum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Plasmodium falciparum | recombinant hydroxymethyldihydropterin pyrophosphokinase-dihydropteroate synthase | - |
7.11 |