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Literature summary for 2.5.1.15 extracted from

  • Kasekarn, W.; Sirawaraporn, R.; Chahomchuen, T.; Cowman, A.F.; Sirawaraporn, W.
    Molecular characterization of bifunctional hydroxymethyldihydropterin pyrophosphokinase-dihydropteroate synthase from Plasmodium falciparum (2004), Mol. Biochem. Parasitol., 137, 43-53.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed under the control of the T5 promoter in a DHPS-deficient Escherichia coli strain Plasmodium falciparum

Inhibitors

Inhibitors Comment Organism Structure
dapsone
-
Plasmodium falciparum
Guanidine HCl 0.25 M, 50% inhibition Plasmodium falciparum
KCl above 0.4 M Plasmodium falciparum
NaCl above 0.2 M Plasmodium falciparum
p-Aminosalicylic acid
-
Plasmodium falciparum
sulfacetamide
-
Plasmodium falciparum
sulfachloropyridazine
-
Plasmodium falciparum
Sulfadiazine
-
Plasmodium falciparum
sulfadimethoxine
-
Plasmodium falciparum
sulfadoxine
-
Plasmodium falciparum
sulfamerazine
-
Plasmodium falciparum
sulfamethoxazole
-
Plasmodium falciparum
sulfamethoxypyridazine
-
Plasmodium falciparum
sulfamoxole
-
Plasmodium falciparum
sulfanilamide
-
Plasmodium falciparum
sulfapyridine
-
Plasmodium falciparum
sulfaquinoxaline
-
Plasmodium falciparum
sulfathiazole
-
Plasmodium falciparum
sulfisoxazole
-
Plasmodium falciparum
Urea 0.9 M, 50% inhibition Plasmodium falciparum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00122
-
2-Amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine
-
Plasmodium falciparum
0.00125
-
4-Aminobenzoate
-
Plasmodium falciparum

Metals/Ions

Metals/Ions Comment Organism Structure
KCl 0.4 M activates Plasmodium falciparum
Mg2+ dependent on Plasmodium falciparum
NaCl 0.2 M activates Plasmodium falciparum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
83000
-
bifunctional hydroxymethyldihydropterin pyrophosphokinase-dihydropteroate synthase, gel filtration Plasmodium falciparum

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant hydroxymethyldihydropterin pyrophosphokinase-dihydropteroate synthase Plasmodium falciparum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0106
-
-
Plasmodium falciparum

Storage Stability

Storage Stability Organism
-20°C and -80°C stable for 3 months in 20 mM Tris–HCl, pH 8.0 buffer containing 10% or 20% glycerol Plasmodium falciparum
-20°C or -80°C, in buffer without glycerol, 30% and 50% of the enzyme activity is lost when stored for 6 months Plasmodium falciparum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine + 4-aminobenzoate
-
Plasmodium falciparum 7,8-dihydropteroate + diphosphate
-
?

Subunits

Subunits Comment Organism
tetramer
-
Plasmodium falciparum

Synonyms

Synonyms Comment Organism
hydroxymethyldihydropterin pyrophosphokinase-dihydropteroate synthase bifunctional enzyme Plasmodium falciparum
pfPPK-DHPS recombinant hydroxymethyldihydropterin pyrophosphokinase-dihydropteroate synthase Plasmodium falciparum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37 45
-
Plasmodium falciparum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 55 30°C: about 40% of maximal activity, 55°C: about 10% of maximal activity Plasmodium falciparum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
inactivation above Plasmodium falciparum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
-
Plasmodium falciparum

pH Range

pH Minimum pH Maximum Comment Organism
8.5 10.5 pH 8.5: about 40% of maximal activity, pH 10.5: about 85% of maximal activity Plasmodium falciparum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0006
-
sulfathiazole
-
Plasmodium falciparum
0.001
-
dapsone
-
Plasmodium falciparum
0.0015
-
sulfachloropyridazine
-
Plasmodium falciparum
0.0016
-
sulfaquinoxaline
-
Plasmodium falciparum
0.0018
-
sulfadimethoxine
-
Plasmodium falciparum
0.0019
-
sulfapyridine
-
Plasmodium falciparum
0.0027
-
sulfamoxole
-
Plasmodium falciparum
0.0033
-
sulfamethoxypyridazine
-
Plasmodium falciparum
0.0044
-
sulfamethoxazole
-
Plasmodium falciparum
0.0048
-
sulfamerazine
-
Plasmodium falciparum
0.0068
-
Sulfadiazine
-
Plasmodium falciparum
0.0076
-
sulfisoxazole
-
Plasmodium falciparum
0.0082
-
p-Aminosalicylic acid
-
Plasmodium falciparum
0.011
-
sulfadoxine
-
Plasmodium falciparum
0.0437
-
sulfanilamide
-
Plasmodium falciparum
0.0941
-
sulfacetamide
-
Plasmodium falciparum

pI Value

Organism Comment pI Value Maximum pI Value
Plasmodium falciparum recombinant hydroxymethyldihydropterin pyrophosphokinase-dihydropteroate synthase
-
7.11