Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5'-methylthioadenosine | - |
Bos taurus | |
5'-methylthioadenosine | - |
Homo sapiens | |
5'-methylthioadenosine | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
S-adenosyl-3-methylthio-1-propylamine | pH 7.4, 37ºC, spleen | Homo sapiens | |
0.08 | - |
1,4-diaminobutane | pH 7.4, 37ºC, spleen | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
cytosol | - |
Bos taurus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
2 * 35000, SDS-PAGE | Homo sapiens |
62000 | - |
spleen, pore gradient gel electrophoresis | Homo sapiens |
62000 | - |
brain, pore gradient gel electrophoresis | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Homo sapiens | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
affinity chromatography | Bos taurus |
ammonium sulfate fractionation, chromatography on DEAE-Cellulose, affinity chromatography, 8700fold purified | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
S-adenosyl 3-(methylsulfanyl)propylamine + putrescine = S-methyl-5'-thioadenosine + spermidine | Michaelis-Menten kinetics | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Homo sapiens | - |
brain | - |
Rattus norvegicus | - |
brain | - |
Bos taurus | - |
kidney | - |
Homo sapiens | - |
kidney | - |
Rattus norvegicus | - |
lung | - |
Homo sapiens | - |
additional information | tissue distribution | Homo sapiens | - |
additional information | tissue distribution | Rattus norvegicus | - |
pancreas | - |
Homo sapiens | - |
pancreas | - |
Rattus norvegicus | - |
placenta | - |
Homo sapiens | - |
spleen | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.66 | - |
- |
Homo sapiens |
Storage Stability | Organism |
---|---|
-80ºC, 20% glycerol, rapid loss of activity in pancreas preparations | Homo sapiens |
-80ºC, 20% glycerol, stable for spleen tissue enzyme during storage and freeze-thawings | Homo sapiens |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Rattus norvegicus |
dimer | - |
Bos taurus |
dimer | 2 * 35000, SDS-PAGE | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
37 | - |
assay at | Rattus norvegicus |
37 | - |
assay at | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rattus norvegicus |
additional information | - |
- |
Bos taurus |
additional information | - |
pI 5.1, in spleen | Homo sapiens |