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Literature summary for 2.5.1.16 extracted from
- Action of decarboxylated S-adenosylmethionine analogs in the spermidine-synthesizing system from rat prostate (1980), Arch. Biochem. Biophys., 201, 241-246.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| cadaverine | weak | Rattus norvegicus |  |
| S-5'-Deoxyadenosyl(5')-2-ethylthioethylamine | uncompetitive with S-adenosyl-3-methylthio-1-propylamine at high concentrations but competitive with S-5'-deoxyadenosyl-5'-3-ethylthiopropylamine | Rattus norvegicus | |
| S-5'-Deoxyadenosyl(5')-2-methylthioethylamine | the most potent inhibitor, competitive with decarboxylated S-adenosylmethionine and S-5'-deoxyadenosyl-(5')-3-ethylthiopropylamine | Rattus norvegicus | |
| S-5'-Deoxyadenosyl(5')-2-thioethylamine | weak | Rattus norvegicus | |
| S-5'-Deoxyadenosyl(5')-3-butylthiopropylamine | weak | Rattus norvegicus | |
| S-5'-deoxyadenosyl(5')-3-thiopropylamine | weak | Rattus norvegicus | |
| S-5'-Deoxyadenosyl(5')-4-methylthiobutylamine | weak | Rattus norvegicus | |
| S-5'-Deoxyadenosyl(5')-4-thiobutylamine | uncompetitive with S-Adenosyl-3-methylthio-1-propylamine | Rattus norvegicus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane | Rattus norvegicus | - |
5'-methylthioadenosine + spermidine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
male Sprague Dawley strain | - |
| Rattus norvegicus Sprague Dawley | - |
male Sprague Dawley strain | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| chromatography on Sephadex G-25 and DEAE-cellulose chromatography | Rattus norvegicus |
| partial | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| prostate gland | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.008 | - |
- |
Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0ºC, 50% loss of activity in 2 weeks | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-5'-deoxyadenosyl-5'-3-butylthiopropylamine + 1,4-diaminobutane | - |
Rattus norvegicus | ? | - |
? | |
| S-5'-deoxyadenosyl-5'-3-ethylthiopropylamine + 1,4-diaminobutane | - |
Rattus norvegicus | ? | - |
? | |
| S-5'-deoxyadenosyl-5'-3-ethylthiopropylamine + 1,4-diaminobutane | - |
Rattus norvegicus Sprague Dawley | ? | - |
? | |
| S-5'-deoxyadenosyl-5'-3-propylthiopropylamine + 1,4-diaminobutane | - |
Rattus norvegicus | ? | - |
? | |
| S-5'-deoxyadenosyl-5'-3-propylthiopropylamine + 1,4-diaminobutane | - |
Rattus norvegicus Sprague Dawley | ? | - |
? | |
| S-adenosyl-(5')-3-methylthio-1-propylamine + 1,4-diamino-2-butene | - |
Rattus norvegicus | N-3-aminopropyl-1,4-diamino-2-butene + 5'-methylthioadenosine | forms with a low yield | ? | |
| S-adenosyl-(5')-3-methylthio-1-propylamine + 1,4-diamino-2-butene | - |
Rattus norvegicus | 5'-methylthioadenosine + N-3-aminopropyl-1,4-diamino-2-butene | in a lower yield than with the natural combination | ? | |
| S-adenosyl-(5')-3-methylthio-1-propylamine + 1,4-diaminobutane | the enzyme transfers only the aminopropyl group, not an aminoethyl or aminobutyl groups | Rattus norvegicus | 5'-methylthioadenosine + spermidine | - |
? | |
| S-adenosyl-(5')-3-methylthio-1-propylamine + 1,4-diaminobutane | sulfonium analogs must carry the aminopropyl group to qualify as a substrate | Rattus norvegicus | 5'-methylthioadenosine + spermidine | - |
? | |
| S-adenosyl-(5')-3-methylthio-1-propylamine + 1,4-diaminobutane | analogues of decarboxylated S-adenosylmethionine act as propylamine donors | Rattus norvegicus | 5'-methylthioadenosine + spermidine | - |
? | |
| S-adenosyl-(5')-3-methylthio-1-propylamine + 1,4-diaminobutane | the enzyme transfers only the aminopropyl group, not an aminoethyl or aminobutyl groups | Rattus norvegicus Sprague Dawley | 5'-methylthioadenosine + spermidine | - |
? | |
| S-adenosyl-(5')-3-methylthio-1-propylamine + 1,4-diaminobutane | sulfonium analogs must carry the aminopropyl group to qualify as a substrate | Rattus norvegicus Sprague Dawley | 5'-methylthioadenosine + spermidine | - |
? | |
| S-adenosyl-(5')-3-methylthio-1-propylamine + 1,4-diaminobutane | analogues of decarboxylated S-adenosylmethionine act as propylamine donors | Rattus norvegicus Sprague Dawley | 5'-methylthioadenosine + spermidine | - |
? | |
| S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane | - |
Rattus norvegicus | 5'-methylthioadenosine + spermidine | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00026 | - |
S-5'-deoxyadenosyl-(5')-2-methylthioethylamine | 0.0035-0.007 mM | Rattus norvegicus | |
| 0.0011 | - |
S-5'-deoxyadenosyl-(5')-2-methylthioethylamine | 0.4 mM | Rattus norvegicus | |
| 0.0015 | - |
S-5'-deoxyadenosyl-(5')-2-methylthioethylamine | 0.3 mM | Rattus norvegicus | |
| 0.0019 | - |
S-5'-deoxyadenosyl-(5')-2-methylthioethylamine | 0.2 mM | Rattus norvegicus | |
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