Literature summary for 2.5.1.16 extracted from

Hibasami, H.; Kawase, M.; Tsukada, T.; Maekawa, S.; Sakurai, M.; Nakashima, K.
2-Mercaptoethylamine, a competitive inhibitor of spermidine synthase in mammalian cells (1988), FEBS Lett., 229, 243-246.

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Inhibitors

Inhibitors	Comment	Organism	Structure
2-mercaptoethylamine	competitive with 1,4-diaminobutane, reversible, its removal restores the enzyme activity, the amino group is necessary to inhibit the enzyme	Rattus norvegicus
5'-ethylthioadenosine	-	Rattus norvegicus
additional information	no inhibition by 2-mercaptoethanol at 0.1 to 1 mM	Rattus norvegicus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.036	-	1,4-diaminobutane	pH 7.5, 37ºC, in the presence of 2-mercaptoethylamine	Rattus norvegicus

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	-	Wistar strain	-
Rattus norvegicus Wistar	-	Wistar strain	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate fractionation, chromatography on DEAE-cellulose, affinity chromatography, 265fold purified	Rattus norvegicus

Source Tissue

Source Tissue	Comment	Organism	Textmining
prostate gland	ventral	Rattus norvegicus	-

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Rattus norvegicus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.005	-	2-mercaptoethylamine	-	Rattus norvegicus

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Release 2023.1 (January 2023)