Literature summary for 2.5.1.16 extracted from

Dufe, V.T.; Luersen, K.; Eschbach, M.L.; Haider, N.; Karlberg, T.; Walter, R.D.; Al-Karadaghi, S.
Cloning, expression, characterisation and three-dimensional structure determination of Caenorhabditis elegans spermidine synthase (2005), FEBS Lett., 579, 6037-6043.

Search for more literature for 2.5.1.16

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Caenorhabditis elegans

Crystallization (Commentary)

Crystallization (Comment)	Organism
-	Caenorhabditis elegans

Inhibitors

Inhibitors	Comment	Organism	Structure
Cyclohexylamine	IC50 2.4 +/- 0.7 microM	Caenorhabditis elegans
trans-4-Methylcyclohexylamine	IC50 7.2 microM	Caenorhabditis elegans

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.111	-	S-adenosylmethioninamine	-	Caenorhabditis elegans
0.158	-	putrescine	-	Caenorhabditis elegans

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
35000	-	calculated	Caenorhabditis elegans
38000	-	SDS-PAGE, including His-tag of 3 kDA	Caenorhabditis elegans

Organism

Organism	UniProt	Comment	Textmining
Caenorhabditis elegans	-	-	-
Caenorhabditis elegans Bristol N2	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Caenorhabditis elegans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.8	-	-	Caenorhabditis elegans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
putrescine + S-adenosylmethioninamine	-	Caenorhabditis elegans	spermidine + 5'-methylthioadenosine	-	?
putrescine + S-adenosylmethioninamine	-	Caenorhabditis elegans Bristol N2	spermidine + 5'-methylthioadenosine	-	?

Subunits

Subunits	Comment	Organism
dimer	gel filtration, molecular weight of 78000	Caenorhabditis elegans

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.0024	-	IC50 2.4 +/- 0.7 microM	Caenorhabditis elegans	Cyclohexylamine
0.0072	-	IC50 7.2 microM	Caenorhabditis elegans	trans-4-Methylcyclohexylamine

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)