Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli, without the first 29 amino acids of the N-terminal extension | Plasmodium falciparum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-Aminooxy-3-aminopropane | poor inhibition | Plasmodium falciparum | |
2-mercaptoethylamine | poor inhibition | Plasmodium falciparum | |
5'-methylthioadenosine | feedback inhibition | Plasmodium falciparum | |
5-amino-1-pentene | 50% inhibition at 6.5 microM | Plasmodium falciparum | |
Cyclohexylamine | 50% inhibition at 19.7 microM | Plasmodium falciparum | |
Dicyclohexylamine | poor inhibition | Plasmodium falciparum | |
additional information | not inhibitory: S-adenosylmethioninamine concentrations up to 150 microM | Plasmodium falciparum | |
trans-4-Methylcyclohexylamine | IC50 1.4 microM | Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0353 | - |
S-adenosylmethioninamine | pH 7.0, 37°C | Plasmodium falciparum | |
0.052 | - |
putrescine | pH 7.0, 37°C | Plasmodium falciparum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Plasmodium falciparum | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
2 * 37000, SDS-PAGE | Plasmodium falciparum |
70000 | - |
gel filtration | Plasmodium falciparum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | Q9NFS5 | - |
- |
Purification (Comment) | Organism |
---|---|
metal affinity chromatography and gel filtration | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
putrescine + S-adenosylmethioninamine | - |
Plasmodium falciparum | spermidine + 5'-methylthioadenosine | - |
? | |
spermidine + S-adenosylmethioninamine | in contrast to mammalian spermidine synthases, spermidine can replace to some extent putrescine as the aminopropyl acceptor | Plasmodium falciparum | spermine + 5'-methylthioadenosine | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 37000, SDS-PAGE | Plasmodium falciparum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00018 | - |
trans-4-Methylcyclohexylamine | - |
Plasmodium falciparum |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0014 | - |
IC50 1.4 microM | Plasmodium falciparum | trans-4-Methylcyclohexylamine |