General Stability | Organism |
---|---|
2-mercaptoethanol stabilizes during purification | Clostridium tetanomorphum |
repeated freeze-thawing inactivates | Clostridium tetanomorphum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diphosphate | - |
Clostridium tetanomorphum | |
EDTA | - |
Clostridium tetanomorphum | |
additional information | no inhibition by phosphate | Clostridium tetanomorphum | |
Trimetaphosphate | - |
Clostridium tetanomorphum | |
tripolyphosphate | - |
Clostridium tetanomorphum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
cyanocob(I)alamin | pH 8.0, 37°C, i.e. vitamin B12, reduced form | Clostridium tetanomorphum | |
0.016 | - |
ATP | pH 8.0, 37°C | Clostridium tetanomorphum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Clostridium tetanomorphum | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activation, less effective than Mn2+ | Clostridium tetanomorphum | |
Mg2+ | activation, less effective than Mn2+ | Clostridium tetanomorphum | |
Mn2+ | - |
Clostridium tetanomorphum | |
additional information | no activation by Ca2+ or Cd2+ | Clostridium tetanomorphum | |
Zn2+ | activation, less effective than Mn2+ | Clostridium tetanomorphum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + cob(I)alamin | Clostridium tetanomorphum | involved in vitamin B12 metabolism | triphosphate + coenzyme B12 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium tetanomorphum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
protamine sulfate, heat treatment and chromatography on Sephadex G-25 and DEAE-cellulose | Clostridium tetanomorphum |
Storage Stability | Organism |
---|---|
-20°C, lyophilized, at least 1 week | Clostridium tetanomorphum |
2-mercaptoethanol stabilizes during storage | Clostridium tetanomorphum |
4°C, about 1 month | Clostridium tetanomorphum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + cob(I)alamin | transfers the adenosyl-group of ATP to the reduced cobalt atom of the cobalamin molecule | Clostridium tetanomorphum | triphosphate + coenzyme B12 | adenosylcobalamin, B12-coenzyme or deoxyadenosyl-B12 | ? | |
ATP + cob(I)alamin | involved in vitamin B12 metabolism | Clostridium tetanomorphum | triphosphate + coenzyme B12 | - |
? | |
cyanocob(I)alamin + ATP | - |
Clostridium tetanomorphum | tripolyphosphate + alpha-(5,6-dimethylbenzimidazolyl)deoxyadenosylcobamide | - |
? | |
additional information | S-adenosylmethionine, vitamin B12r or B12a, AMP, ADP are no substrates | Clostridium tetanomorphum | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Clostridium tetanomorphum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
no appreciable loss of activity after 10 min | Clostridium tetanomorphum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
phosphate or Tris buffer | Clostridium tetanomorphum |