General Stability | Organism |
---|---|
DTT required to maintain activity | Salmonella enterica subsp. enterica serovar Typhimurium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0028 | - |
ATP | pH 8.0, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.0052 | - |
cob(I)alamin | pH 8.0, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
K+ | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
Mg2+ | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
Mn2+ | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
2 * 25000, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
42000 | - |
non-denaturing PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | - |
structural gene cob A | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation followed by two chromatographic steps | Salmonella enterica subsp. enterica serovar Typhimurium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.053 | - |
pH 8.0, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium |
0.053 | - |
activity at final purification step | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + cob(I)alamin | - |
Salmonella enterica subsp. enterica serovar Typhimurium | triphosphate + coenzyme B12 | adenosylcobalamin, B12-coenzyme or deoxyadenosyl-B12 | ? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 25000, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
stable for several weeks with dithiothreitol | Salmonella enterica subsp. enterica serovar Typhimurium |
55 | - |
sharp decrease in activity with preincubation at 55°C or higher | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |