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Literature summary for 2.5.1.17 extracted from

  • Stich, T.A.; Buan, N.R.; Escalante-Semerena, J.C.; Brunold, T.C.
    Spectroscopic and computational studies of the ATP:corrinoid adenosyltransferase (CobA) from Salmonella enterica: insights into the mechanism of adenosylcobalamin biosynthesis (2005), J. Am. Chem. Soc., 127, 8710-8719.
    View publication on PubMed

General Stability

General Stability Organism
dithiothreitol stabilizes the enzyme Salmonella enterica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + cob(I)alamin Salmonella enterica mechanism of adenosylcobalamin biosynthesis triphosphate + adenosylcob(III)alamin
-
?
additional information Salmonella enterica the flavodoxin in vivo reducing agent that serves as the electron donor to the enzyme possesses a reduction potential that is considerably more positive than that of the Co2+/1+ couple of the corrinoid substrate, the enzyme overcomes this challenge by formation of an ATP-activated unique paramagnetic Co2+ corrinoid species by partial conversion, overview ?
-
?

Organism

Organism UniProt Comment Textmining
Salmonella enterica
-
-
-

Oxidation Stability

Oxidation Stability Organism
enzyme is oxygen-labile Salmonella enterica

Reaction

Reaction Comment Organism Reaction ID
2 ATP + 2 cob(II)alamin + a reduced flavoprotein = 2 triphosphate + 2 adenosylcob(III)alamin + an oxidized flavoprotein reaction mechanism, substrate structure Salmonella enterica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.02
-
purified recombinant enzyme Salmonella enterica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + cob(I)alamin mechanism of adenosylcobalamin biosynthesis Salmonella enterica triphosphate + adenosylcob(III)alamin
-
?
ATP + cob(I)alamin formation of the the essential C-Co bond by transferring the adenosyl group from a molecule of ATP to a transient Co1+ corrinoid species generated by the active site Salmonella enterica triphosphate + adenosylcob(III)alamin
-
?
additional information the flavodoxin in vivo reducing agent that serves as the electron donor to the enzyme possesses a reduction potential that is considerably more positive than that of the Co2+/1+ couple of the corrinoid substrate, the enzyme overcomes this challenge by formation of an ATP-activated unique paramagnetic Co2+ corrinoid species by partial conversion, overview Salmonella enterica ?
-
?
additional information enzyme-substrate interactions, intermediate/transition structures, spectroscopic mechanism analysis, computational modeling, overview Salmonella enterica ?
-
?

Synonyms

Synonyms Comment Organism
ATP:corrinoid adenosyltransferase
-
Salmonella enterica
CobA
-
Salmonella enterica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Salmonella enterica