Activating Compound | Comment | Organism | Structure |
---|---|---|---|
coenzyme B12 | i.e. adenosylcob(III)alamin, is required for stimulation of eut operon expression | Salmonella enterica | |
coenzyme B12 | i.e. adenosylcob(III)alamin, is required for stimulation of eut operon expression | Salmonella enterica subsp. enterica serovar Typhimurium |
Cloned (Comment) | Organism |
---|---|
2 enzymes encoded by genes eutT and cobA, DNA sequence determination, analysis of several geno- and phenotypic strains, enzyme is expressed e.g. as fusion protein or to complement a deficient strain, overview | Salmonella enterica |
2 enzymes encoded by genes eutT and cobA, DNA sequence determination, analysis of several geno- and phenotypic strains, enzyme is expressed e.g. as fusion protein or to complement a deficient strain, overview | Salmonella enterica subsp. enterica serovar Typhimurium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
bathophenanthroline | inhibition of EutT | Salmonella enterica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | required and contained by EutT | Salmonella enterica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + cob(I)alamin | Salmonella enterica | last step in the corrinoid adenosylation pathway, EutT and CobA | triphosphate + adenosylcob(III)alamin | - |
ir | |
ATP + cob(I)alamin | Salmonella enterica subsp. enterica serovar Typhimurium | last step in the corrinoid adenosylation pathway, EutT and CobA | triphosphate + adenosylcob(III)alamin | - |
ir | |
additional information | Salmonella enterica | EutT is necessary and sufficient for growth of a cobA eutT strain on ethanolamine as a carbon and energy or nitrogen source | ? | - |
? | |
additional information | Salmonella enterica subsp. enterica serovar Typhimurium | EutT is necessary and sufficient for growth of a cobA eutT strain on ethanolamine as a carbon and energy or nitrogen source | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica | - |
EutT; 2 enzymes encoded by genes eutT and cobA with different primary sequences, cobA is a housekeeping gene, several derivatives of strain TR6583, several geno- and phenotypes, overview | - |
Salmonella enterica subsp. enterica serovar Typhimurium | P31570 | CobA; 2 enzymes encoded by genes eutT and cobA with different primary sequences, cobA is a housekeeping gene, several derivatives of strain TR6583, several geno- and phenotypes, overview | - |
Oxidation Stability | Organism |
---|---|
EutT is oxygen-labile, exposure to air leads to complete loss of activity | Salmonella enterica |
EutT is oxygen-labile, exposure to air leads to complete loss of activity | Salmonella enterica subsp. enterica serovar Typhimurium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0061 | - |
strain carrying the complementation plasmd eutT+, in vivo activity | Salmonella enterica subsp. enterica serovar Typhimurium |
0.0061 | - |
strain carrying the complementation plasmid eutT+, in vivo activity | Salmonella enterica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + cob(I)alamin | last step in the corrinoid adenosylation pathway, EutT and CobA | Salmonella enterica | triphosphate + adenosylcob(III)alamin | - |
ir | |
ATP + cob(I)alamin | last step in the corrinoid adenosylation pathway, EutT and CobA | Salmonella enterica subsp. enterica serovar Typhimurium | triphosphate + adenosylcob(III)alamin | - |
ir | |
ATP + cob(I)alamin | CobA has an ATP-binding P-loop motif, while EutT has a cysteine-rich region reminiscent of a conserved S-adenosylmethionine Fe-S cluster motif | Salmonella enterica | triphosphate + adenosylcob(III)alamin | - |
ir | |
ATP + cob(I)alamin | CobA has an ATP-binding P-loop motif, while EutT has a cysteine-rich region reminiscent of a conserved S-adenosylmethionine Fe-S cluster motif | Salmonella enterica subsp. enterica serovar Typhimurium | triphosphate + adenosylcob(III)alamin | - |
ir | |
additional information | EutT is necessary and sufficient for growth of a cobA eutT strain on ethanolamine as a carbon and energy or nitrogen source | Salmonella enterica | ? | - |
? | |
additional information | EutT is necessary and sufficient for growth of a cobA eutT strain on ethanolamine as a carbon and energy or nitrogen source | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ATP:co(I)rrinoid adenosyltransferase | - |
Salmonella enterica |
ATP:co(I)rrinoid adenosyltransferase | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
ATP:corrinoid adenosyltransferase | - |
Salmonella enterica |
ATP:corrinoid adenosyltransferase | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
CobA | - |
Salmonella enterica |
CobA | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
cobalamin adenosyltransferase | - |
Salmonella enterica |
cobalamin adenosyltransferase | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
EutT | - |
Salmonella enterica |
EutT | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Salmonella enterica |
25 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Salmonella enterica |
8 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |