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Literature summary for 2.5.1.18 extracted from

  • Yeung, T.C.; Gidari, A.S.
    Purification and properties of a chicken liver glutathione S-transferase (1980), Arch. Biochem. Biophys., 205, 404-411.
    View publication on PubMed
Search for more literature for 2.5.1.18

Inhibitors

Inhibitors Comment Organism Structure
hemin competitive with 1-chloro-2,4-dinitrobenzene, non-competitive with glutathione Gallus gallus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.35
-
 reduced glutathione
-
 Gallus gallus
1.92
-
 1-chloro-2,4-dinitrobenzene
-
 Gallus gallus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Gallus gallus 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
 2 * 23000, SDS-PAGE Gallus gallus
45000
-
 gel filtration Gallus gallus

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
liver foetal Gallus gallus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.03
-
 with 1-chloro-2,4-dinitrobenzene + glutathione or trans-4-phenyl-3-buten-2-one as substrate Gallus gallus
0.49
-
 with 1,2-epoxy-3-(4-nitrophenoxy)propane as substrate Gallus gallus
0.75
-
 with 4-nitrobenzyl chloride as substrate Gallus gallus
0.83
-
 with ethacrynic acid as substrate Gallus gallus
3.44
-
-
 Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + 1,2-dichloro-4-nitrobenzene
-
 Gallus gallus ?
-
 ?
glutathione + 1,2-epoxy-3-(4-nitrophenoxy)propane
-
 Gallus gallus ?
-
 ?
glutathione + 1-chloro-2,4-dinitrobenzene
-
 Gallus gallus S-2,4-dinitrophenylglutathione + HCl
-
 ?
glutathione + 4-nitrobenzyl chloride
-
 Gallus gallus ?
-
 ?
glutathione + ethacrynic acid
-
 Gallus gallus ?
-
 ?
glutathione + trans-4-phenyl-3-buten-2-one
-
 Gallus gallus ?
-
 ?
additional information obligate requirement for reduced glutathione as thiol donor Gallus gallus ?
-
 ?
RX + glutathione RX: R: aliphatic, aromatic or heterocyclic, X: sulfate, nitrite or halide, enzyme also catalyzes: the addition of aliphatic epoxides and arene oxides to glutathione, the reduction of polyol nitrate by glutathione to polyol and nitrite, certain isomerization reactions and disulfide interchange Gallus gallus HX + R-S-glutathione
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 23000, SDS-PAGE Gallus gallus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 10 min, 50% loss of activity Gallus gallus
80
-
 complete inactivation after 10 min Gallus gallus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.8
-
-
 Gallus gallus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.001
-
 hemin with reduced glutathione Gallus gallus