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Literature summary for 2.5.1.18 extracted from

  • Deng, F.; Hatzios, K.K.
    Purification and characterization of two glutathione S-transferase isozymes from Indica-type rice involved in herbicide detoxification (2002), Pestic. Biochem. Physiol., 72, 10-23.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
bromosulfophthalein
-
Oryza sativa
chlorotriphenyltin
-
Oryza sativa
Cibacron blue
-
Oryza sativa
iodoacetate
-
Oryza sativa
S-hexylglutathione
-
Oryza sativa
tridiphane
-
Oryza sativa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
1 * 25000 + 1 * 28000, GST II-III, SDS-PAGE Oryza sativa
28000
-
1 * 25000 + 1 * 28000, GST II-III, SDS-PAGE Oryza sativa
30000
-
2 * 30000, GST I, SDS-PAGE Oryza sativa
45000 60000 gel filtration Oryza sativa

Organism

Organism UniProt Comment Textmining
Oryza sativa
-
rice, v. Tequing, indica
-

Purification (Commentary)

Purification (Comment) Organism
homogeneity, 2 isoenzymes: GST I-I and GST II-III Oryza sativa

Source Tissue

Source Tissue Comment Organism Textmining
shoot etiolated Oryza sativa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
82.27
-
GST I-I Oryza sativa
93.21
-
GST II-III Oryza sativa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + 1-chloro-2,4-dinitrobenzene
-
Oryza sativa S-2,4-dinitrophenylglutathione + HCl
-
?
glutathione + cumene hydroperoxide
-
Oryza sativa ?
-
?
glutathione + ethacrynic acid
-
Oryza sativa ?
-
?
glutathione + fenchlorim
-
Oryza sativa ?
-
?
glutathione + metolachlor
-
Oryza sativa ?
-
?
glutathione + petrilachlor
-
Oryza sativa ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 30000, GST I, SDS-PAGE Oryza sativa
dimer 1 * 25000 + 1 * 28000, GST II-III, SDS-PAGE Oryza sativa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
GST I-I Oryza sativa
7
-
GST II-III Oryza sativa