Protein Variants | Comment | Organism |
---|---|---|
D150A | turnover-number for reaction with reduced glutathione and 1-chloro-2,4-dinitrobenzene is 1.3fold higher than wild-type value. KM-value for 1-chloro-2,4-dinitrobenzene is 2fold higher than wild-type value | Anopheles dirus |
D150S | turnover-number for reaction with reduced glutathione and 1-chloro-2,4-dinitrobenzene is 1.1fold higher than wild-type value. KM-value for 1-chloro-2,4-dinitrobenzene is 2fold higher than wild-type value | Anopheles dirus |
D150Y | turnover-number for reaction with reduced glutathione and 1-chloro-2,4-dinitrobenzene is 1.2fold higher than wild-type value. KM-value for 1-chloro-2,4-dinitrobenzene is 1.7fold higher than wild-type value | Anopheles dirus |
H144A | turnover-number for reaction with reduced glutathione and 1-chloro-2,4-dinitrobenzene is 1.2fold higher than wild-type value. KM-value for 1-chloro-2,4-dinitrobenzene is nearly identical to wild-type value | Anopheles dirus |
R96A | turnover-number for reaction with reduced glutathione and 1-chloro-2,4-dinitrobenzene is 1.9fold higher than wild-type value. KM-value for 1-chloro-2,4-dinitrobenzene is 1.5fold higher than wild-type value | Anopheles dirus |
V147A | turnover-number for reaction with reduced glutathione and 1-chloro-2,4-dinitrobenzene nearly identical to wild-type value. KM-value for 1-chloro-2,4-dinitrobenzene is 1.5fold lower than wild-type value | Anopheles dirus |
V147L | turnover-number for reaction with reduced glutathione and 1-chloro-2,4-dinitrobenzene is 1.2fold lower than wild-type value. KM-value for 1-chloro-2,4-dinitrobenzene is 1.8fold lower than wild-type value | Anopheles dirus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,2-dichloro-4-nitrobenzene | 1 mM, 53.3% inhibition of wild-type enzyme, 40.4% of mutant enzyme H144A, 42.4% of mutant enzyme V147A, 54.3% of mutant enzyme V147L, 35.9% of mutant enzyme R96A | Anopheles dirus | |
4-nitrobenzyl chloride | 1 mM, 50.5% inhibition of wild-type enzyme, 43.9% of mutant enzyme H144A, 40.8% of mutant enzyme V147A, 47.9% of mutant enzyme V147L, 30.8% of mutant enzyme R96A | Anopheles dirus | |
4-nitrophenethyl bromide | 0.1 mM, 40.1% inhibition of wild-type enzyme, 23.6% of mutant enzyme H144A, 19.5% of mutant enzyme V147A, 31.4% of mutant enzyme V147L, 27.3% of mutant enzyme R96A | Anopheles dirus | |
cumene hydroperoxide | 2.5 mM, 43.3% inhibition of wild-type enzyme, 27% of mutant enzyme H144A, 50.6% of mutant enzyme V147A, 41.5% of mutant enzyme V147L, 48.1% of mutant enzyme R96A | Anopheles dirus | |
deltamethrin | 0.01 mM, 65.4% inhibition of wild-type enzyme, 44.2% of mutant enzyme H144A, 59.5% of mutant enzyme V147A, 66.1% of mutant enzyme V147L, 88.6% of mutant enzyme R96A | Anopheles dirus | |
Ethacrynic acid | 0.001 mM, 35.3% inhibition of wild-type enzyme, 32.7% of mutant enzyme H144A, 27.8% of mutant enzyme V147A, 23.0% of mutant enzyme V147L, 29.1% of mutant enzyme R96A | Anopheles dirus | |
permethrin | 0.01 mM, 51.1% inhibition of wild-type enzyme, 38.8% of mutant enzyme H144A, 53.1% of mutant enzyme V147A, 49% of mutant enzyme V147L, 65.5% of mutant enzyme R96A | Anopheles dirus | |
S-hexyl glutathione | 0.01 mM, 21.7% inhibition of wild-type enzyme, 23% of mutant enzyme H144A, 10.8% of mutant enzyme V147A, 24.2% of mutant enzyme V147L, 14.7% of mutant enzyme R96A | Anopheles dirus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
1-chloro-2,4-dinitrobenzene | 25-27°C, pH 6.5, mutant enzyme D150S | Anopheles dirus | |
0.08 | - |
1-chloro-2,4-dinitrobenzene | 25-27°C, pH 6.5, mutant enzyme D150A | Anopheles dirus | |
0.09 | - |
1-chloro-2,4-dinitrobenzene | 25-27°C, pH 6.5, mutant enzyme D150Y | Anopheles dirus | |
0.13 | - |
1-chloro-2,4-dinitrobenzene | 25-27°C, pH 6.5, wild-type enzyme | Anopheles dirus | |
0.14 | - |
1-chloro-2,4-dinitrobenzene | 25-27°C, pH 6.5, mutant enzyme H144A | Anopheles dirus | |
0.17 | - |
reduced glutathione | 25-27°C, pH 6.5, mutant enzyme D150S | Anopheles dirus | |
0.19 | - |
1-chloro-2,4-dinitrobenzene | 25-27°C, pH 6.5, mutant enzyme V147A | Anopheles dirus | |
0.2 | - |
1-chloro-2,4-dinitrobenzene | 25-27°C, pH 6.5, mutant enzyme V147L | Anopheles dirus | |
0.21 | - |
reduced glutathione | 25-27°C, pH 6.5, mutant enzyme D150Y | Anopheles dirus | |
0.26 | - |
reduced glutathione | 25-27°C, pH 6.5, mutant enzyme V147A | Anopheles dirus | |
0.29 | - |
reduced glutathione | 25-27°C, pH 6.5, wild-type enzyme | Anopheles dirus | |
0.33 | - |
reduced glutathione | 25-27°C, pH 6.5, mutant enzyme D150A | Anopheles dirus | |
0.34 | - |
reduced glutathione | 25-27°C, pH 6.5, mutant enzyme V147L | Anopheles dirus | |
0.37 | - |
reduced glutathione | 25-27°C, pH 6.5, mutant enzyme H144A | Anopheles dirus | |
0.38 | - |
1-chloro-2,4-dinitrobenzene | 25-27°C, pH 6.5, mutant enzyme R96A | Anopheles dirus | |
2.75 | - |
reduced glutathione | 25-27°C, pH 6.5, mutant enzyme R96A | Anopheles dirus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Anopheles dirus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
reduced glutathione + 1-chloro-2,4-dinitrobenzene | - |
Anopheles dirus | S-2,4-dinitrophenylglutathione + HCl | - |
? | |
reduced glutathione + 4-nitrobenzyl chloride | - |
Anopheles dirus | ? | - |
? | |
reduced glutathione + 4-nitrophenethyl bromide | - |
Anopheles dirus | ? | - |
? | |
reduced glutathione + ethacrynic acid | - |
Anopheles dirus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glutathione S-transferase AdFSTD3-3 | - |
Anopheles dirus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
21.6 | - |
1-chloro-2,4-dinitrobenzene | 25-27°C, pH 6.5, mutant enzyme V147L | Anopheles dirus | |
21.6 | - |
reduced glutathione | 25-27°C, pH 6.5, mutant enzyme V147L | Anopheles dirus | |
24.9 | - |
1-chloro-2,4-dinitrobenzene | 25-27°C, pH 6.5, mutant enzyme V147A | Anopheles dirus | |
24.9 | - |
reduced glutathione | 25-27°C, pH 6.5, mutant enzyme V147A | Anopheles dirus | |
25.2 | - |
1-chloro-2,4-dinitrobenzene | 25-27°C, pH 6.5, wild-type enzyme | Anopheles dirus | |
25.2 | - |
reduced glutathione | 25-27°C, pH 6.5, wild-type enzyme | Anopheles dirus | |
27.8 | - |
1-chloro-2,4-dinitrobenzene | 25-27°C, pH 6.5, mutant enzyme D150S | Anopheles dirus | |
27.8 | - |
reduced glutathione | 25-27°C, pH 6.5, mutant enzyme D150S | Anopheles dirus | |
29.1 | - |
1-chloro-2,4-dinitrobenzene | 25-27°C, pH 6.5, mutant enzyme H144A | Anopheles dirus | |
29.1 | - |
reduced glutathione | 25-27°C, pH 6.5, mutant enzyme H144A | Anopheles dirus | |
29.5 | - |
1-chloro-2,4-dinitrobenzene | 25-27°C, pH 6.5, mutant enzyme D150Y | Anopheles dirus | |
29.5 | - |
reduced glutathione | 25-27°C, pH 6.5, mutant enzyme D150Y | Anopheles dirus | |
31.7 | - |
1-chloro-2,4-dinitrobenzene | 25-27°C, pH 6.5, mutant enzyme D150A | Anopheles dirus | |
31.7 | - |
reduced glutathione | 25-27°C, pH 6.5, mutant enzyme D150A | Anopheles dirus | |
46.8 | - |
1-chloro-2,4-dinitrobenzene | 25-27°C, pH 6.5, mutant enzyme R96A | Anopheles dirus | |
46.8 | - |
reduced glutathione | 25-27°C, pH 6.5, mutant enzyme R96A | Anopheles dirus |