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Literature summary for 2.5.1.18 extracted from
- Purification and biochemical properties of glutathione S-transferase from Lactuca sativa (2005), J. Biochem. Mol. Biol., 38, 232-237.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| benastatin A | IC50: 0.00041 mM | Lactuca sativa |  |
| Ethacrynic acid | 0.00028 mM | Lactuca sativa | |
| S-(2,4-dinitrophenyl)glutathione | IC50: 0.0034 mM | Lactuca sativa | |
| S-hexyl-GSH | IC50: 0.0152 mM | Lactuca sativa | |
| S-methyl-GSH | IC50: 0.0123 mM | Lactuca sativa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23000 | - |
2 * 23000, SDS-PAGE | Lactuca sativa |
| 48000 | - |
gel filtration | Lactuca sativa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lactuca sativa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Lactuca sativa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.03 | - |
- |
Lactuca sativa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,2-epoxy-3-(p-nitrophenoxy)propane + glutathione | - |
Lactuca sativa | ? | - |
? | |
| 1-chloro-2,3-dinitrobenzene + glutathione | - |
Lactuca sativa | S-(2,3-dinitrophenyl)glutathione + HCl | - |
? | |
| ethacrynic acid + glutathione | - |
Lactuca sativa | ? | - |
? | |
| additional information | the enzyme also exhibits glutathione peroxidase activity towards cumene hydroperoxide | Lactuca sativa | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 23000, SDS-PAGE | Lactuca sativa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
10 min, stable up to | Lactuca sativa |
| 50 | - |
10 min, about 40% loss of activity | Lactuca sativa |
| 60 | - |
10 min, about 75% loss of activity | Lactuca sativa |
| 65 | - |
10 min, about 90% loss of activity | Lactuca sativa |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.00041 | - |
IC50: 0.00041 mM | Lactuca sativa | benastatin A | |
| 0.0034 | - |
IC50: 0.0034 mM | Lactuca sativa | S-(2,4-dinitrophenyl)glutathione | |
| 0.0123 | - |
IC50: 0.0123 mM | Lactuca sativa | S-methyl-GSH | |
| 0.0152 | - |
IC50: 0.0152 mM | Lactuca sativa | S-hexyl-GSH | |
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