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Literature summary for 2.5.1.18 extracted from

  • Adewale, I.O.; Afolayan, A.
    Purification and catalytic properties of glutathione transferase from the hepatopancreas of crayfish Macrobrachium vollenhovenii (herklots) (2004), J. Biochem. Mol. Toxicol., 18, 332-344.
    View publication on PubMed
Search for more literature for 2.5.1.18

General Stability

General Stability Organism
low guanidine-HCl concentrations, less than 0.5 M, denature the enzyme, complete renaturation is possible. At higher concentrations of the denaturant, 0.5-4 M, complete renaturation is not achieved. The extent of renaturation is a function of protein concentration Macrobrachium vollenhovenii

Inhibitors

Inhibitors Comment Organism Structure
1-chloro-2,3-dinitrobenzene
-
 Macrobrachium vollenhovenii
1-chloro-2,4-dinitrobenzene
-
 Macrobrachium vollenhovenii
Cibacron blue IC50: 10 nM Macrobrachium vollenhovenii
GSH
-
 Macrobrachium vollenhovenii
Hematin IC50: 0.005 mM Macrobrachium vollenhovenii
NEM IC50: 33 mM Macrobrachium vollenhovenii
S-hexylglutathione IC50: 0.34 mM Macrobrachium vollenhovenii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.31
-
 GSH
-
 Macrobrachium vollenhovenii
2.03
-
 1-chloro-2,4-dinitrobenzene
-
 Macrobrachium vollenhovenii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24100
-
 2 * 24100, SDS-PAGE Macrobrachium vollenhovenii
46000
-
 gel filtration Macrobrachium vollenhovenii

Organism

Organism UniProt Comment Textmining
Macrobrachium vollenhovenii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Macrobrachium vollenhovenii

Renatured (Commentary)

Renatured (Comment) Organism
low guanidine-HCl concentrations, less than 0.5 M, denature the enzyme, complete renaturation is possible. At higher concentrations of the denaturant, 0.5-4 M, complete renaturation is not achieved. The extent of renaturation is a function of protein concentration Macrobrachium vollenhovenii

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12.36
-
-
 Macrobrachium vollenhovenii

Storage Stability

Storage Stability Organism
-20°C, 10% glycerol, stable for 4 months Macrobrachium vollenhovenii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-chloro-2,4-dinitrobenzene + GSH
-
 Macrobrachium vollenhovenii S-(2,4-dinitrophenyl)glutathione + HCl
-
 ?
7-chloro-4-nitrobenzo-2-oxa-1,3-diazole + GSH
-
 Macrobrachium vollenhovenii ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 24100, SDS-PAGE Macrobrachium vollenhovenii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
20.3
-
 GSH
-
 Macrobrachium vollenhovenii
20.3
-
 1-chloro-2,4-dinitrobenzene
-
 Macrobrachium vollenhovenii

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.09
-
 1-chloro-2,4-dinitrobenzene
-
 Macrobrachium vollenhovenii
1.13
-
 GSH
-
 Macrobrachium vollenhovenii

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00001
-
 IC50: 10 nM Macrobrachium vollenhovenii Cibacron blue
0.005
-
 IC50: 0.005 mM Macrobrachium vollenhovenii Hematin
0.34
-
 IC50: 0.34 mM Macrobrachium vollenhovenii S-hexylglutathione
33
-
 IC50: 33 mM Macrobrachium vollenhovenii NEM