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Literature summary for 2.5.1.18 extracted from

  • Labrou, N.E.; Kotzia, G.A.; Clonis, Y.D.
    Engineering the xenobiotic substrate specificity of maize glutathione S-transferase I (2004), Protein Eng. Des. Sel., 17, 741-748.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
F35L turnover number with 1-chloro-2,4-dinitrobenzoate is 83.9% of the wild-type value, the ratio of turnover number to KM-value is 5.6% of the wild-type value, turnover number with ethacrynic acid is 65% of the wild-type value Zea mays
I118F turnover number with 1-chloro-2,4-dinitrobenzoate is 55% of the wild-type value, the ratio of turnover number to KM-value is 419% of the wild-type value, turnover number with ethacrynic acid is 54% of the wild-type value Zea mays
W12I turnover number with 1-chloro-2,4-dinitrobenzoate is 47.7% of the wild-type value, the ratio of turnover number to KM-value is 11.1% of the wild-type value, turnover number with ethacrynic acid is 35% of the wild-type value Zea mays
W12P turnover number with 1-chloro-2,4-dinitrobenzoate is 7.6% of the wild-type value, the ratio of turnover number to KM-value is 3.7% of the wild-type value, turnover number with ethacrynic acid is 9.5% of the wild-type value Zea mays

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00006
-
Ethacrynic acid mutant enzyme I118F Zea mays
0.0005
-
Ethacrynic acid wild-type enzyme GST I Zea mays
0.0008
-
Ethacrynic acid mutant enzyme T12P Zea mays
0.00094
-
Ethacrynic acid mutant enzyme T12I Zea mays
0.0019
-
Ethacrynic acid mutant enzyme F35L Zea mays
0.0125
-
GSH mutant enzyme T12P, reaction with ethacrynic acid Zea mays
0.0406
-
GSH mutant enzyme F35L, reaction with ethacrynic acid Zea mays
0.0608
-
GSH mutant enzyme T12I, reaction with ethacrynic acid Zea mays
0.0726
-
GSH mutant enzyme I118F, reaction with ethacrynic acid Zea mays
0.0755
-
GSH wild-type enzyme GST I, reaction with ethacrynic acid Zea mays
0.1
-
GSH mutant enzyme T12P, reaction with 1-chloro-2,3-dinitrobenzoate Zea mays
0.21
-
1-chloro-2,3-dinitrobenzoate mutant enzyme I118F Zea mays
0.8
-
GSH mutant enzyme T12I, reaction with 1-chloro-2,3-dinitrobenzoate Zea mays
0.9
-
GSH mutant enzyme I118F, reaction with 1-chloro-2,3-dinitrobenzoate Zea mays
1.1
-
GSH wild-type enzyme GST I, reaction with 1-chloro-2,3-dinitrobenzoate Zea mays
1.6
-
1-chloro-2,3-dinitrobenzoate wild-type enzyme GST I Zea mays
3.5
-
GSH mutant enzyme F35L, reaction with 1-chloro-2,3-dinitrobenzoate Zea mays
3.5
-
1-chloro-2,3-dinitrobenzoate mutant enzyme T12P Zea mays
6.9
-
1-chloro-2,3-dinitrobenzoate mutant enzyme T12I Zea mays
20.7
-
1-chloro-2,3-dinitrobenzoate mutant enzyme F35L Zea mays

Organism

Organism UniProt Comment Textmining
Zea mays
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-chloro-2,4-dinitrobenzene + glutathione
-
Zea mays S-(2,4-dinitrophenyl)glutathione + HCl
-
?
ethacrynic acid + glutathione
-
Zea mays ?
-
?
glutathione + 1-chloro-2,3-dinitrobenzoate
-
Zea mays ?
-
?

Synonyms

Synonyms Comment Organism
glutathione S-transferase I
-
Zea mays

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information
-
Zea mays