Any feedback?
Literature summary for 2.5.1.18 extracted from
- Expression and characterization of a sigma-class glutathione S-transferase of the fall webworm, Hyphantria cunea (2007), Biosci. Biotechnol. Biochem., 71, 553-560.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene gst, DNA and amino acid sequence determination and analysis, phylogenetic tree, sequence comparisons, overexpression of soluble enzyme in Escherichia coli | Hyphantria cunea |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| deltamethrin | an insecticide | Hyphantria cunea |  |
| fenitrothion | an insecticide | Hyphantria cunea | |
| permethrin | an insecticide | Hyphantria cunea | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Hyphantria cunea | |
| 0.41 | - |
1-chloro-2,4-dinitrobenzene | pH 8.0, 30°C, recombinant enzyme | Hyphantria cunea | |
| 0.53 | - |
4-hydroxynonenal | pH 8.0, 30°C, recombinant enzyme | Hyphantria cunea | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23204 | - |
x * 23204, sequence calculation | Hyphantria cunea |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hyphantria cunea | Q4ACU7 | sigma class isozyme, gene gst; fall webworm, gene gst, sigma class isozyme | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli to homogeneity | Hyphantria cunea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| larva | four-instar larvae | Hyphantria cunea | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione + 1-chloro-2,4-dinitrobenzene | - |
Hyphantria cunea | chloride + 2,4-dinitrophenyl-glutathione | - |
? | |
| glutathione + 4-hydroxynonenal | - |
Hyphantria cunea | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 23204, sequence calculation | Hyphantria cunea |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glutathione S-transferase | - |
Hyphantria cunea |
| GST | - |
Hyphantria cunea |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Hyphantria cunea |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
30 min, purified recombinant enzyme, stable | Hyphantria cunea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Hyphantria cunea |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 11 | purified recombinant enzyme, 75% remaining activity | Hyphantria cunea |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Hyphantria cunea | sequence calculation | - |
8.75 |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
