Application | Comment | Organism |
---|---|---|
drug development | the enzyme is a target for drug development against the parasite | Plasmodium vivax |
Cloned (Comment) | Organism |
---|---|
gene gst, DNA and amino acid sequence determination and analysis, expression of wild-type enzyme in Escherichia coli strain DH5alpha, expression of His-tagged enzyme in Escherichia coli strain M15 | Plasmodium vivax |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
glutathione | pH 8.0, 37°C, recombinant enzyme | Plasmodium vivax | |
2.1 | - |
1-chloro-2,4-dinitrobenzene | pH 8.0, 37°C, recombinant enzyme | Plasmodium vivax |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
2 * 25000, recombinant enzyme, SDS-PAGE | Plasmodium vivax |
50000 | - |
recombinant enzyme, gel filtration | Plasmodium vivax |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Plasmodium vivax | the enzyme is essential for parasite survival by protecting the parasite against oxidative stress and buffering the detoxification of heme-binding compounds | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium vivax | Q0ZS46 | gene gst | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain M15 by nickel affinity chromatography, recombinant enzyme from Escherichia coli strain DH5alpha by gel filtration | Plasmodium vivax |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.21 | - |
purified recombinant enzyme, substrate ethacrynic acid | Plasmodium vivax |
0.58 | - |
purified recombinant enzyme, substrate 1,2-dichloro-4-nitrobenzene | Plasmodium vivax |
34.7 | - |
purified recombinant enzyme, substrate 1-chloro-2,4-dinitrobenzene | Plasmodium vivax |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + 1,2-dichloro-4-nitrobenzene | low activity compared to 1-chloro-2,4-dinitrobenzene | Plasmodium vivax | ? | - |
? | |
glutathione + 1-chloro-2,4-dinitrobenzene | preferred substrate | Plasmodium vivax | chloride + 2,4-dinitrophenyl-glutathione | - |
? | |
glutathione + ethacrynic acid | low activity compared to 1-chloro-2,4-dinitrobenzene | Plasmodium vivax | ? | - |
? | |
additional information | the enzyme is essential for parasite survival by protecting the parasite against oxidative stress and buffering the detoxification of heme-binding compounds | Plasmodium vivax | ? | - |
? | |
additional information | 2-nitrophenyl acetate, bromosulfophthalein, and cumene hydroperoxide are poor substrates, overview | Plasmodium vivax | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 25000, recombinant enzyme, SDS-PAGE | Plasmodium vivax |
Synonyms | Comment | Organism |
---|---|---|
glutathione S-transferase | - |
Plasmodium vivax |
GST | - |
Plasmodium vivax |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Plasmodium vivax |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
purified recombinant enzyme, quite stable | Plasmodium vivax |
37 | - |
purified recombinant enzyme, rapid decrease in activity | Plasmodium vivax |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
recombinant enzyme | Plasmodium vivax |