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Literature summary for 2.5.1.18 extracted from

  • Fortin, P.D.; Horsman, G.P.; Yang, H.M.; Eltis, L.D.
    A glutathione S-transferase catalyzes the dehalogenation of inhibitory metabolites of polychlorinated biphenyls (2006), J. Bacteriol., 188, 4424-4430.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene bphK, expression in Escherichia coli strain GJ1158 Paraburkholderia xenovorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information steady-state kinetics using 1-chloro-2,4-dinitrobenzene as substrate Paraburkholderia xenovorans
0.11
-
glutathione pH 6.5, 25°C, recombinant enzyme, with (2Z,4E)-3-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid Paraburkholderia xenovorans
0.37
-
glutathione pH 6.5, 25°C, recombinant enzyme, with (2E,4Z)-5-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid Paraburkholderia xenovorans
0.39
-
glutathione pH 6.5, 25°C, recombinant enzyme, with (2Z,4E)-3-chloro-6-(3,5-dichlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid Paraburkholderia xenovorans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glutathione + (2E,4Z)-5-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid Paraburkholderia xenovorans
-
chloride + 5-glutathionyl-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
?
glutathione + (2Z,4E)-3-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid Paraburkholderia xenovorans
-
chloride + 3-glutathionyl-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
?
glutathione + (2Z,4E)-3-chloro-6-(3,5-dichlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid Paraburkholderia xenovorans
-
?
-
?
glutathione + 1-chloro-2,4-dinitrobenzene Paraburkholderia xenovorans
-
chloride + 2,4-dinitrophenyl-glutathione
-
?
additional information Paraburkholderia xenovorans the enzyme catalyzes the dehalogenation of inhibitory metabolites of polychlorinated biphenyls and may be responsible for facilitation of PCB degradation by the bph pathway, overview ?
-
?

Organism

Organism UniProt Comment Textmining
Paraburkholderia xenovorans
-
gene bphK
-

Purification (Commentary)

Purification (Comment) Organism
recombinant BphK from Escherichia coli strain GJ1158 by glutathione affinity chromatography Paraburkholderia xenovorans

Reaction

Reaction Comment Organism Reaction ID
RX + glutathione = HX + R-S-glutathione the enzyme uses a rapid-equilibrium random-order ternary-complex mechanism or a compulsory order ternary-complex mechanism Paraburkholderia xenovorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + (2E,4Z)-5-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
Paraburkholderia xenovorans chloride + 5-glutathionyl-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
?
glutathione + (2Z,4E)-3-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
Paraburkholderia xenovorans chloride + 3-glutathionyl-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
?
glutathione + (2Z,4E)-3-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid preferred polychlorinated biphenyl metabolite substrate Paraburkholderia xenovorans chloride + 3-glutathionyl-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
?
glutathione + (2Z,4E)-3-chloro-6-(3,5-dichlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid
-
Paraburkholderia xenovorans ?
-
?
glutathione + 1-chloro-2,4-dinitrobenzene
-
Paraburkholderia xenovorans chloride + 2,4-dinitrophenyl-glutathione
-
?
additional information the enzyme catalyzes the dehalogenation of inhibitory metabolites of polychlorinated biphenyls and may be responsible for facilitation of PCB degradation by the bph pathway, overview Paraburkholderia xenovorans ?
-
?

Synonyms

Synonyms Comment Organism
BphK
-
Paraburkholderia xenovorans
glutathione S-transferase
-
Paraburkholderia xenovorans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Paraburkholderia xenovorans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.01
-
glutathione pH 6.5, 25°C, recombinant enzyme, with (2Z,4E)-3-chloro-6-(3,5-dichlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid Paraburkholderia xenovorans
0.16
-
glutathione pH 6.5, 25°C, recombinant enzyme, with (2Z,4E)-3-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid Paraburkholderia xenovorans
0.24
-
glutathione pH 6.5, 25°C, recombinant enzyme, with (2E,4Z)-5-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid Paraburkholderia xenovorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
assay at Paraburkholderia xenovorans