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Literature summary for 2.5.1.18 extracted from
- Molecular cloning and characterization of a senescence-induced tau-class glutathione S-transferase from barley leaves (2005), Plant Cell Physiol., 46, 1540-1548.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the tau class isozyme SIGST is senescence-induced, overview | Hordeum vulgare |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| isozyme SIGST, DNA and amino acid sequence determination and analysis | Hordeum vulgare |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.285 | - |
glutathione | pH 8.3 | Hordeum vulgare |  |
| 0.293 | - |
1-chloro-2,4-dinitrobenzene | pH 8.3 | Hordeum vulgare | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Hordeum vulgare | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26700 | - |
2 * 26700, isozyme SIGST, SDS-PAGE | Hordeum vulgare |
| 49000 | - |
isozyme SIGST, gel filtration | Hordeum vulgare |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hordeum vulgare | Q3MUP2 | isozyme SIGST; tau class isozyme SIGST | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native isozyme SIGST 19fold to homogeneity by ammonium sulfate fractionation, hydrophobic interaction and anion exchange chromatography, and gel filtration | Hordeum vulgare |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Hordeum vulgare | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.127 | 1.66 | purified enzyme | Hordeum vulgare |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione + 1-chloro-2,4-dinitrobenzene | - |
Hordeum vulgare | chloride + 2,4-dinitrophenyl-glutathione | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 26700, isozyme SIGST, SDS-PAGE | Hordeum vulgare |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glutathione S-transferase | - |
Hordeum vulgare |
| GST | - |
Hordeum vulgare |
| SIGST | - |
Hordeum vulgare |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.3 | - |
isozyme SIGST | Hordeum vulgare |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9 | isozyme SIGST | Hordeum vulgare |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Hordeum vulgare | sequence calculation | - |
6.35 |
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