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Literature summary for 2.5.1.18 extracted from

  • Rhee, J.S.; Lee, Y.M.; Hwang, D.S.; Won, E.J.; Raisuddin, S.; Shin, K.H.; Lee, J.S.
    Molecular cloning, expression, biochemical characteristics, and biomarker potential of theta class glutathione S-transferase (GST-T) from the polychaete Neanthes succinea (2007), Aquat. Toxicol., 83, 104-115.
    View publication on PubMed
Search for more literature for 2.5.1.18

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Alitta succinea

Inhibitors

Inhibitors Comment Organism Structure
Cibacron blue
-
 Alitta succinea
additional information not inhibited by haematin and N-ethylmaleimade Alitta succinea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5
-
 1-chloro-2,4-dinitrobenzene at 25°C Alitta succinea
3.11
-
 glutathione at 25°C Alitta succinea

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ copper exposure (0.072 mg/l) causes an up-regulation of GST-T expression Alitta succinea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26660
-
 calculated from amino acid sequence Alitta succinea
30000
-
 His-tagged enzyme, SDS-PAGE Alitta succinea

Organism

Organism UniProt Comment Textmining
Alitta succinea A6MN06
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni+-NTA column chromatography Alitta succinea

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.082
-
 recombinant enzyme, at 25°C Alitta succinea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + 1-chloro-2,4-dinitrobenzene
-
 Alitta succinea S-(2,4-dinitrophenyl)glutathione + HCl
-
 ?

Synonyms

Synonyms Comment Organism
GST-T
-
 Alitta succinea
theta class glutathione S-transferase
-
 Alitta succinea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Alitta succinea

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
 50 80-85% of the activity can be retained in temperature range from 0°C to 50°C Alitta succinea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Alitta succinea

pI Value

Organism Comment pI Value Maximum pI Value
Alitta succinea theoretical value
-
 8.52