Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain C41 | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
C10A | shows a 5 or 6fold higher kcat than wild type for both glutathione and 1-chloro-2,4-dinitrobenzene, accompanied by an 8fold increase in the Km for glutathione | Escherichia coli |
C10S | shows a 25fold lower kcat for glutathione H and 5fold lower kcat for 1-chloro-2,4-dinitrobenzene than wild type accompanied by a 6fold decrease in the Km for glutathione | Escherichia coli |
H106A | shows clearly decreased activity compared to the wild type enzyme | Escherichia coli |
H106F | shows decreased activity compared to the wild type enzyme | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
glutathione | mutant enzyme C10S, at 25°C, in 100 mM phosphate buffer (pH 6.5) | Escherichia coli | |
0.3 | - |
glutathione | wild type enzyme, at 25°C, in 100 mM phosphate buffer (pH 6.5) | Escherichia coli | |
1.3 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme H106A, at 25°C, in 100 mM phosphate buffer (pH 6.5) | Escherichia coli | |
1.4 | - |
glutathione | mutant enzyme H106A, at 25°C, in 100 mM phosphate buffer (pH 6.5) | Escherichia coli | |
1.9 | - |
glutathione | mutant enzyme H106F, at 25°C, in 100 mM phosphate buffer (pH 6.5) | Escherichia coli | |
1.9 | - |
1-chloro-2,4-dinitrobenzene | wild type enzyme, at 25°C, in 100 mM phosphate buffer (pH 6.5) | Escherichia coli | |
2.1 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme H106F, at 25°C, in 100 mM phosphate buffer (pH 6.5) | Escherichia coli | |
2.5 | - |
glutathione | mutant enzyme C10A, at 25°C, in 100 mM phosphate buffer (pH 6.5) | Escherichia coli | |
4.3 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme C10A, at 25°C, in 100 mM phosphate buffer (pH 6.5) | Escherichia coli | |
4.4 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme C10S, at 25°C, in 100 mM phosphate buffer (pH 6.5) | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0A9D2 | strain TG2 | - |
Purification (Comment) | Organism |
---|---|
nickel affinity resin chromatography | Escherichia coli |
Renatured (Comment) | Organism |
---|---|
the denaturation process is fully reversible at pH 7.5 | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + 1-chloro-2,4-dinitrobenzene | - |
Escherichia coli | S-(2,4-dinitrophenyl)-glutathione + HCl | - |
? |
Synonyms | Comment | Organism |
---|---|---|
EGST | - |
Escherichia coli |
glutathione S-transferase | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
glutathione | mutant enzyme C10S, at 25°C, in 100 mM phosphate buffer (pH 6.5) | Escherichia coli | |
1.1 | - |
glutathione | mutant enzyme H106A, at 25°C, in 100 mM phosphate buffer (pH 6.5) | Escherichia coli | |
1.1 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme H106A, at 25°C, in 100 mM phosphate buffer (pH 6.5) | Escherichia coli | |
1.6 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme C10S, at 25°C, in 100 mM phosphate buffer (pH 6.5) | Escherichia coli | |
4.8 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme H106F, at 25°C, in 100 mM phosphate buffer (pH 6.5) | Escherichia coli | |
6.8 | - |
glutathione | mutant enzyme H106F, at 25°C, in 100 mM phosphate buffer (pH 6.5) | Escherichia coli | |
7.7 | - |
glutathione | wild type enzyme, at 25°C, in 100 mM phosphate buffer (pH 6.5) | Escherichia coli | |
9.1 | - |
1-chloro-2,4-dinitrobenzene | wild type enzyme, at 25°C, in 100 mM phosphate buffer (pH 6.5) | Escherichia coli | |
41 | - |
glutathione | mutant enzyme C10A, at 25°C, in 100 mM phosphate buffer (pH 6.5) | Escherichia coli | |
55.8 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme C10A, at 25°C, in 100 mM phosphate buffer (pH 6.5) | Escherichia coli |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
most stable at pH 7.5 | Escherichia coli |