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Literature summary for 2.5.1.18 extracted from

  • Ivarsson, Y.; Norrgard, M.A.; Hellman, U.; Mannervik, B.
    Engineering the enantioselectivity of glutathione transferase by combined active-site mutations and chemical modifications (2007), Biochim. Biophys. Acta, 1770, 1374-1381.
    View publication on PubMed
Search for more literature for 2.5.1.18

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
C115A mutant displays significant decrease in 1-chloro-2,4-dinitrobenzene activity Homo sapiens
C174A mutant displays significant decrease in 1-chloro-2,4-dinitrobenzene activity Homo sapiens
C78A mutant displays significant decrease in 1-chloro-2,4-dinitrobenzene activity Homo sapiens
C88A mutant displays significant decrease in 1-chloro-2,4-dinitrobenzene activity Homo sapiens
G112C mutant displays as high catalytic activity as wild type GST M1-1 Homo sapiens
I10A/G112C mutant displays significant decrease in 1-chloro-2,4-dinitrobenzene activity Homo sapiens
I10C mutant displays significant decrease in 1-chloro-2,4-dinitrobenzene activity Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.035
-
 (R,R)-trans-stilbene oxide wild type enzyme G112C, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
0.06
-
 (R,R)-trans-stilbene oxide wild type enzyme I10C, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
0.08
-
 (S,S)-trans-stilbene oxide wild type enzyme G112C, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
0.1
-
 (R,R)-trans-stilbene oxide wild type enzyme, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
0.11
-
 (S,S)-trans-stilbene oxide wild type enzyme, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
0.12
-
 (S,S)-trans-stilbene oxide wild type enzyme I10C, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
0.8
-
 (R,R)-1-phenylpropylene oxide wild type enzyme I10C, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
0.9
-
 (R,R)-1-phenylpropylene oxide wild type enzyme G112C, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
1.3
-
 (S)-styrene-7,8-oxide mutant enzyme I10C, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
1.6
-
 (S)-styrene-7,8-oxide mutant enzyme G112C, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
2.2
-
 (S,S)-1-phenylpropylene oxide wild type enzyme, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
2.4
-
 (S,S)-1-phenylpropylene oxide wild type enzyme I10C, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
2.4
-
 (R,R)-1-phenylpropylene oxide wild type enzyme, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
3.8
-
 (S,S)-1-phenylpropylene oxide wild type enzyme G112C, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
4.2
-
 (R)-styrene-7,8-oxide mutant enzyme G112C, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
4.7
-
 (R)-styrene-7,8-oxide wild type enzyme, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
4.9
-
 (S)-styrene-7,8-oxide wild type enzyme, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
8.8
-
 (R)-styrene-7,8-oxide mutant enzyme I10C, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
GSH-affinity column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + (R)-styrene-7,8-oxide
-
 Homo sapiens ?
-
 ?
glutathione + (R,R)-1-phenylpropylene oxide
-
 Homo sapiens ?
-
 ?
glutathione + (R,R)-trans-stilbene oxide
-
 Homo sapiens ?
-
 ?
glutathione + (S)-styrene-7,8-oxide
-
 Homo sapiens ?
-
 ?
glutathione + (S,S)-1-phenylpropylene oxide
-
 Homo sapiens ?
-
 ?
glutathione + (S,S)-trans-stilbene oxide
-
 Homo sapiens ?
-
 ?
glutathione + 1-chloro-2,4-dinitrobenzene
-
 Homo sapiens S-(2,4-dinitrophenyl)glutathione + HCl
-
 ?

Subunits

Subunits Comment Organism
homodimer x-ray crystallography Homo sapiens

Synonyms

Synonyms Comment Organism
glutathione transferase M1-1
-
 Homo sapiens
GST M1-1 originally called GST mu Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.07
-
 (S,S)-trans-stilbene oxide wild type enzyme I10C, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
0.2
-
 (S,S)-trans-stilbene oxide wild type enzyme G112C, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
0.37
-
 (R,R)-trans-stilbene oxide wild type enzyme I10C, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
0.46
-
 (S,S)-trans-stilbene oxide wild type enzyme, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
0.83
-
 (R,R)-1-phenylpropylene oxide mutant enzyme I10C, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
1.1
-
 (S,S)-1-phenylpropylene oxide wild type enzyme I10C, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
1.3
-
 (R,R)-trans-stilbene oxide wild type enzyme G112C, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
2.3
-
 (S)-styrene-7,8-oxide mutant enzyme I10C, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
2.4
-
 (S,S)-1-phenylpropylene oxide wild type enzyme G112C, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
2.7
-
 (R)-styrene-7,8-oxide mutant enzyme G112C, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
3.2
-
 (S)-styrene-7,8-oxide mutant enzyme G112C, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
4.4
-
 (R)-styrene-7,8-oxide mutant enzyme I10C, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
4.6
-
 (R,R)-1-phenylpropylene oxide mutant enzyme G112C, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
4.8
-
 (R)-styrene-7,8-oxide wild type enzyme, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
7.7
-
 (R,R)-trans-stilbene oxide wild type enzyme, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
8.4
-
 (S)-styrene-7,8-oxide wild type enzyme, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
9
-
 (S,S)-1-phenylpropylene oxide wild type enzyme, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens
17.7
-
 (R,R)-1-phenylpropylene oxide wild type enzyme, at 30°C in 250 mM Tris-HCl buffer, pH 7.2 Homo sapiens