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Literature summary for 2.5.1.18 extracted from

  • Huang, Y.C.; Misquitta, S.; Blond, S.Y.; Adams, E.; Colman, R.F.
    Catalytically active monomer of glutathione S-transferase pi and key residues involved in the electrostatic interaction between subunits (2008), J. Biol. Chem., 283, 32880-32888.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.5.1.18

Cloned(Commentary)

Cloned (Comment) Organism
mutant enzymes expressed in Escherichia coli Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method Homo sapiens

Protein Variants

Protein Variants Comment Organism
D90N greatly decreased specific activity, shows no appreciable changes in Km for 1-chloro-2,4-dinitrobenzene and has similar circular dichroism spectra to that of wild type enzyme, the dimer-monomer equilibrium is shifted toward monomer Homo sapiens
D94N greatly decreased specific activity, shows no appreciable changes in Km for 1-chloro-2,4-dinitrobenzene and has similar circular dichroism spectra to that of wild type enzyme, the dimer-monomer equilibrium is shifted toward monomer Homo sapiens
R70Q greatly decreased specific activity, shows no appreciable changes in Km for 1-chloro-2,4-dinitrobenzene and has similar circular dichroism spectra to that of wild type enzyme, the dimer-monomer equilibrium is shifted toward monomer Homo sapiens
R74Q greatly decreased specific activity, shows no appreciable changes in Km for 1-chloro-2,4-dinitrobenzene and has similar circular dichroism spectra to that of wild type enzyme, the dimer-monomer equilibrium is shifted toward monomer Homo sapiens
T67A increased specific activity Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.25
-
 glutathione mutant enzyme T67A, in phosphate buffer (pH 6.5), at 25°C Homo sapiens
0.31
-
 glutathione wild type enzyme, in phosphate buffer (pH 6.5), at 25°C Homo sapiens
0.64
-
 1-chloro-2,4-dinitrobenzene mutant enzyme D94N, in phosphate buffer (pH 6.5), at 25°C Homo sapiens
0.73
-
 glutathione mutant enzyme D90N, in phosphate buffer (pH 6.5), at 25°C Homo sapiens
0.88
-
 glutathione mutant enzyme R74Q, in phosphate buffer (pH 6.5), at 25°C Homo sapiens
0.9
-
 1-chloro-2,4-dinitrobenzene mutant enzyme R74Q, in phosphate buffer (pH 6.5), at 25°C Homo sapiens
1
-
 1-chloro-2,4-dinitrobenzene wild type enzyme, in phosphate buffer (pH 6.5), at 25°C Homo sapiens
1.11
-
 1-chloro-2,4-dinitrobenzene mutant enzyme R70Q, in phosphate buffer (pH 6.5) ,at 25°C Homo sapiens
1.26
-
 1-chloro-2,4-dinitrobenzene mutant enzyme T67A, in phosphate buffer (pH 6.5), at 25°C Homo sapiens
1.62
-
 1-chloro-2,4-dinitrobenzene mutant enzyme D90N, in phosphate buffer (pH 6.5), at 25°C Homo sapiens
4.1
-
 glutathione mutant enzyme R70Q, in phosphate buffer (pH 6.5), at 25°C Homo sapiens
52
-
 glutathione mutant enzyme D94N, in phosphate buffer (pH 6.5), at 25°C Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
 2 * 23000, X-ray crystallography Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P09211
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.019
-
 mutant enzyme D90N, in phosphate buffer (pH 6.5), at 25°C Homo sapiens
0.18
-
 mutant enzyme R74Q, in phosphate buffer (pH 6.5), at 25°C Homo sapiens
0.21
-
 mutant enzyme D94N, in phosphate buffer (pH 6.5), at 25°C Homo sapiens
3.4
-
 mutant enzyme R70Q, in phosphate buffer (pH 6.5), at 25°C Homo sapiens
75
-
 wild type enzyme, in phosphate buffer (pH 6.5) Homo sapiens
102
-
 mutant enzyme T67A, in phosphate buffer (pH 6.5), at 25°C Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + 1-chloro-2,4-dinitrobenzene
-
 Homo sapiens S-(2,4-dinitrophenyl)glutathione + HCl
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 23000, X-ray crystallography Homo sapiens

Synonyms

Synonyms Comment Organism
glutathione S-transferase pi
-
 Homo sapiens
GST P1-1
-
 Homo sapiens
GST pi
-
 Homo sapiens