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Literature summary for 2.5.1.18 extracted from

  • Travensolo, R.F.; Garcia, W.; Muniz, J.R.; Caruso, C.S.; Lemos, E.G.; Carrilho, E.; Araujo, A.P.
    Cloning, expression, purification and characterization of recombinant glutathione-S-transferase from Xylella fastidiosa (2008), Protein Expr. Purif., 59, 153-160.
    View publication on PubMed
Search for more literature for 2.5.1.18

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Xylella fastidiosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
 2 * 28000, in solution, gel filtration Xylella fastidiosa
56000
-
 gel filtration Xylella fastidiosa

Organism

Organism UniProt Comment Textmining
Xylella fastidiosa Q87E36
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Xylella fastidiosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.34
-
 crude extract, in 0.1 M potassium phosphate buffer (pH 6.5), at 25°C Xylella fastidiosa
3.3
-
 after 9.7fold purification, in 0.1 M potassium phosphate buffer (pH 6.5), at 25°C Xylella fastidiosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + 1-chloro-2,4-dinitrobenzene
-
 Xylella fastidiosa S-(2,4-dinitrophenyl)glutathione + HCl
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 28000, in solution, gel filtration Xylella fastidiosa

Synonyms

Synonyms Comment Organism
glutathione-S-transferase
-
 Xylella fastidiosa
GST
-
 Xylella fastidiosa