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Literature summary for 2.5.1.18 extracted from

  • Whalen, K.E.; Morin, D.; Lin, C.Y.; Tjeerdema, R.S.; Goldstone, J.V.; Hahn, M.E.
    Proteomic identification, cDNA cloning and enzymatic activity of glutathione S-transferases from the generalist marine gastropod, Cyphoma gibbosum (2008), Arch. Biochem. Biophys., 478, 7-17.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene CgGSTM1, DNA and amino acid sequence determination and analysis, phylogenetic analysis Cyphoma gibbosum
gene CgGSTM2, DNA and amino acid sequence determination and analysis, phylogenetic analysis Cyphoma gibbosum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Cyphoma gibbosum 5829
-

Organism

Organism UniProt Comment Textmining
Cyphoma gibbosum A7LFK0 isozyme CgGSTM2; gene CgGSTM2
-
Cyphoma gibbosum A7LFK1 isozyme CgGSTM1; gene CgGSTM1
-

Purification (Commentary)

Purification (Comment) Organism
native cytosolic isozyme 220fold from digestive glands by ultracentrifugation, gel filtration, and glutathione affinity chromatography Cyphoma gibbosum

Source Tissue

Source Tissue Comment Organism Textmining
digestive gland from individuals fed for 4 days on one of six gorgonian diets, overview Cyphoma gibbosum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
75
-
purified native CgGSTM1 Cyphoma gibbosum
75
-
purified native CgGSTM2 Cyphoma gibbosum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + 1-chloro-2,4-dinitrobenzene
-
Cyphoma gibbosum S-(2,4-dinitrophenyl)glutathione + HCl
-
?

Synonyms

Synonyms Comment Organism
CgGSTM1
-
Cyphoma gibbosum
CgGSTM2
-
Cyphoma gibbosum
glutathione S-transferase
-
Cyphoma gibbosum
GST
-
Cyphoma gibbosum
More two major GST subunits are mu-class GSTs, one minor GST subunit is a putative theta-class GST Cyphoma gibbosum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Cyphoma gibbosum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Cyphoma gibbosum