Cloned (Comment) | Organism |
---|---|
gene CgGSTM1, DNA and amino acid sequence determination and analysis, phylogenetic analysis | Cyphoma gibbosum |
gene CgGSTM2, DNA and amino acid sequence determination and analysis, phylogenetic analysis | Cyphoma gibbosum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Cyphoma gibbosum | 5829 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cyphoma gibbosum | A7LFK0 | isozyme CgGSTM2; gene CgGSTM2 | - |
Cyphoma gibbosum | A7LFK1 | isozyme CgGSTM1; gene CgGSTM1 | - |
Purification (Comment) | Organism |
---|---|
native cytosolic isozyme 220fold from digestive glands by ultracentrifugation, gel filtration, and glutathione affinity chromatography | Cyphoma gibbosum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
digestive gland | from individuals fed for 4 days on one of six gorgonian diets, overview | Cyphoma gibbosum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
75 | - |
purified native CgGSTM1 | Cyphoma gibbosum |
75 | - |
purified native CgGSTM2 | Cyphoma gibbosum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + 1-chloro-2,4-dinitrobenzene | - |
Cyphoma gibbosum | S-(2,4-dinitrophenyl)glutathione + HCl | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CgGSTM1 | - |
Cyphoma gibbosum |
CgGSTM2 | - |
Cyphoma gibbosum |
glutathione S-transferase | - |
Cyphoma gibbosum |
GST | - |
Cyphoma gibbosum |
More | two major GST subunits are mu-class GSTs, one minor GST subunit is a putative theta-class GST | Cyphoma gibbosum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Cyphoma gibbosum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Cyphoma gibbosum |