Cloned (Comment) | Organism |
---|---|
a synthetic GST gene based on the wild type amino acid sequence, changing the second residue to an isoleucine, is overexpressed in Escherichia coli strain BL21 to obtain GST as a soluble protein | Penaeus vannamei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | binds in the GSH-binding site by coordination with Asp and Gln residues, molecular modeling, overview | Penaeus vannamei | |
Cu2+ | - |
Penaeus vannamei |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the residues that coordinate the metal ion in the shrimp GST are N59, Q72 from one monomer and D106 from the partner chain | Penaeus vannamei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Penaeus vannamei | Q49SB0 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant Ile2 enzyme variant from Escherichia coli strain BL21 by glutathione affinity chromatography | Penaeus vannamei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + 1-chloro-2,4-dinitrobenzene | - |
Penaeus vannamei | S-(2,4-dinitrophenyl)glutathione + HCl | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glutathione S-transferase | - |
Penaeus vannamei |
GST | - |
Penaeus vannamei |
mu class glutathione S-transferase | - |
Penaeus vannamei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Penaeus vannamei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Penaeus vannamei |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00039 | - |
- |
Penaeus vannamei | Cd2+ | |
0.00477 | - |
- |
Penaeus vannamei | Cu2+ |
General Information | Comment | Organism |
---|---|---|
physiological function | glutathione S-transferases are a family of detoxifying enzymes that catalyze the conjugation of glutathione to electrophiles, thereby increasing the solubility of xenobiotics and aiding its excretion from the cell | Penaeus vannamei |