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Literature summary for 2.5.1.18 extracted from

  • Fedulova, N.; Raffalli-Mathieu, F.; Mannervik, B.
    Characterization of porcine alpha-class glutathione transferase A1-1 (2011), Arch. Biochem. Biophys., 507, 205-211.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene GSTA1, from ovary and testis, DNA and amino acid sequence determination and analysis, expression of isozyme GST A1-1 in Escherichia coli strain Rosetta(DE3) Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.121
-
phenethyl isothiocyanate pH 7.8, 30°C Sus scrofa
0.16
-
trans-2-nonenal pH 7.8, 30°C Sus scrofa
0.2
-
1-chloro-2,4-dinitrobenzene pH 7.8, 30°C Sus scrofa
0.237
-
5-androstene-3,17-dione pH 7.8, 30°C Sus scrofa
0.7
-
5-Pregnene-3,20-dione pH 7.8, 30°C Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa P51781 gene GSTA1
-
Sus scrofa Yorkshire pig P51781 gene GSTA1
-

Purification (Commentary)

Purification (Comment) Organism
recombinant isozyme GST A1-1 from Escherichia coli by ammonium sulfate fractionation, gel filtration, S-hexylglutathione affinity chromatography, ultrafiltration, and again gel filtration Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
adipose tissue
-
Sus scrofa
-
liver
-
Sus scrofa
-
additional information tissue distribution of the GSTA1 mRNA, overview Sus scrofa
-
ovary
-
Sus scrofa
-
pituitary gland
-
Sus scrofa
-
testis
-
Sus scrofa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-chloro-2,4-dinitrobenzene + glutathione
-
Sus scrofa S-(2,4-dinitrophenyl)glutathione + HCl
-
?
1-chloro-2,4-dinitrobenzene + glutathione
-
Sus scrofa Yorkshire pig S-(2,4-dinitrophenyl)glutathione + HCl
-
?
5-androstene-3,17-dione + glutathione
-
Sus scrofa ?
-
?
5-androstene-3,17-dione + glutathione
-
Sus scrofa Yorkshire pig ?
-
?
5-pregnene-3,20-dione + glutathione
-
Sus scrofa ?
-
?
5-pregnene-3,20-dione + glutathione
-
Sus scrofa Yorkshire pig ?
-
?
additional information the enzyme is active with cumene hydroperoxide. The GST A1-1 catalytic activity in steroid isomerization reactions is at least 10fold lower than the corresponding values for porcine GST A2-2, whereas the activity with 1-chloro-2,4-dinitrobenzene is approximately 8fold higher. Differences in the H-site residues of mammalian Alpha-class GSTs may explain the catalytic divergence Sus scrofa ?
-
?
additional information the enzyme is active with cumene hydroperoxide. The GST A1-1 catalytic activity in steroid isomerization reactions is at least 10fold lower than the corresponding values for porcine GST A2-2, whereas the activity with 1-chloro-2,4-dinitrobenzene is approximately 8fold higher. Differences in the H-site residues of mammalian Alpha-class GSTs may explain the catalytic divergence Sus scrofa Yorkshire pig ?
-
?
phenethyl isothiocyanate + glutathione
-
Sus scrofa ?
-
?
phenethyl isothiocyanate + glutathione
-
Sus scrofa Yorkshire pig ?
-
?
trans-2-nonenal + glutathione
-
Sus scrofa ?
-
?

Synonyms

Synonyms Comment Organism
alpha-class glutathione transferase
-
Sus scrofa
glutathione transferase A1-1
-
Sus scrofa
GST A1-1
-
Sus scrofa
pGSTA1
-
Sus scrofa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Sus scrofa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.01
-
5-Pregnene-3,20-dione pH 7.8, 30°C Sus scrofa
0.155
-
trans-2-nonenal pH 7.8, 30°C Sus scrofa
4.8
-
5-androstene-3,17-dione pH 7.8, 30°C Sus scrofa
9.5
-
phenethyl isothiocyanate pH 7.8, 30°C Sus scrofa
84
-
1-chloro-2,4-dinitrobenzene pH 7.8, 30°C Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
assay at Sus scrofa

pI Value

Organism Comment pI Value Maximum pI Value
Sus scrofa GST A1-1
-
9

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1
-
trans-2-nonenal pH 7.8, 30°C Sus scrofa
16
-
5-Pregnene-3,20-dione pH 7.8, 30°C Sus scrofa
20
-
5-androstene-3,17-dione pH 7.8, 30°C Sus scrofa
79
-
phenethyl isothiocyanate pH 7.8, 30°C Sus scrofa
420
-
1-chloro-2,4-dinitrobenzene pH 7.8, 30°C Sus scrofa