Cloned (Comment) | Organism |
---|---|
recombinant expression of wild-type and mutannt isozymes GSTU4-4 | Glycine max |
Protein Variants | Comment | Organism |
---|---|---|
N48A | site-directed mutagensiss of a strictly conserved residue, the mutation significantly affects substrate binding and specificity. Mutation of Asn48 and Pro49 residues may bring about secondary effects altering the thermal stability and the catalytic activity (kcat) of the enzyme without affecting the nature of the rate-limiting step of the catalytic reaction | Glycine max |
P49A | site-directed mutagensiss of a strictly conserved residue, the mutation significantly affects substrate binding and specificity. Mutation of Asn48 and Pro49 residues may bring about secondary effects altering the thermal stability and the catalytic activity (kcat) of the enzyme without affecting the nature of the rate-limiting step of the catalytic reaction | Glycine max |
S13A | site-directed mutagensiss of a strictly conserved residue, the mutation significantly affects substrate binding and specificity | Glycine max |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetic analysis of wild-type and mutannt isozymes GSTU4-4, overview | Glycine max | |
0.14 | - |
1-chloro-2,4-dinitrobenzene | pH 6.5, 30°C, recombinant GSTU4-4 mutant P49A | Glycine max | |
0.158 | - |
1-chloro-2,4-dinitrobenzene | pH 6.5, 30°C, recombinant wild-type GSTU4-4 | Glycine max | |
0.159 | - |
glutathione | pH 6.5, 30°C, recombinant wild-type GSTU4-4 | Glycine max | |
0.26 | - |
1-chloro-2,4-dinitrobenzene | pH 6.5, 30°C, recombinant GSTU4-4 mutant D48A | Glycine max | |
0.29 | - |
glutathione | pH 6.5, 30°C, recombinant GSTU4-4 mutant D48A | Glycine max | |
0.35 | - |
glutathione | pH 6.5, 30°C, recombinant GSTU4-4 mutant S13A | Glycine max | |
0.47 | - |
1-chloro-2,4-dinitrobenzene | pH 6.5, 30°C, recombinant GSTU4-4 mutant S13A | Glycine max | |
0.5 | - |
glutathione | pH 6.5, 30°C, recombinant GSTU4-4 mutant P49A | Glycine max |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | O49235 | isozyme GSTU4-4 | - |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutannt isozymes GSTU4-4 by glutathione affinity chromatography | Glycine max |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
RX + glutathione = HX + R-S-glutathione | catalytic mechanism, ovverview | Glycine max |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-chloro-2,4-dinitrobenzene + glutathione | residue Ser13 is directly involved in the reaction chemistry and the correct positioning of GSH and CDNB in the ternary catalytic complex. Asn48 and Pro49 have a direct role on the structural integrity of the GSH-binding site (G-site) | Glycine max | 2,4-dinitrophenyl-glutathione + HCl | - |
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Subunits | Comment | Organism |
---|---|---|
More | secondary structure of GmGSTU4-4 from PDB ID 2VO4 and sequence comparisons, overview | Glycine max |
Synonyms | Comment | Organism |
---|---|---|
GmGSTU4-4 | - |
Glycine max |
tau class glutathione transferase | - |
Glycine max |
tau class GSTU4-4 | - |
Glycine max |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Glycine max |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
purified recommbinant wild-type and mutant isozymes GSTU4-4, 100 mM potassium phosphate, pH 7, stable up to | Glycine max |
57.1 | - |
Tm for the Pro49Ala mutant | Glycine max |
60.7 | - |
Tm for the Asn48Ala mutant | Glycine max |
64.8 | - |
Tm for the Ser13Ala mutant | Glycine max |
68.4 | - |
Tm for the wild-type isozyme GSTU4-4 | Glycine max |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0897 | - |
1-chloro-2,4-dinitrobenzene | pH 6.5, 30°C, recombinant GSTU4-4 mutant S13A | Glycine max | |
1.45 | - |
1-chloro-2,4-dinitrobenzene | pH 6.5, 30°C, recombinant GSTU4-4 mutant D48A | Glycine max | |
2.48 | - |
1-chloro-2,4-dinitrobenzene | pH 6.5, 30°C, recombinant wild-type GSTU4-4 | Glycine max | |
5.9 | - |
1-chloro-2,4-dinitrobenzene | pH 6.5, 30°C, recombinant GSTU4-4 mutant P49A | Glycine max |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Glycine max |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.2 | 8.5 | activity range, profile overview | Glycine max |
General Information | Comment | Organism |
---|---|---|
additional information | role of conserved residues Ser13, Asn48 and Pro49 in the catalytic mechanism of the tau class glutathione transferase, overview | Glycine max |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.19 | - |
1-chloro-2,4-dinitrobenzene | pH 6.5, 30°C, recombinant GSTU4-4 mutant S13A | Glycine max | |
5.58 | - |
1-chloro-2,4-dinitrobenzene | pH 6.5, 30°C, recombinant GSTU4-4 mutant D48A | Glycine max | |
15.7 | - |
1-chloro-2,4-dinitrobenzene | pH 6.5, 30°C, recombinant wild-type GSTU4-4 | Glycine max | |
42.1 | - |
1-chloro-2,4-dinitrobenzene | pH 6.5, 30°C, recombinant GSTU4-4 mutant P49A | Glycine max |