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Literature summary for 2.5.1.18 extracted from

  • Axarli, I.; Georgiadou, C.; Dhavala, P.; Papageorgiou, A.; Labrou, N.
    Investigation of the role of conserved residues Ser13, Asn48 and Pro49 in the catalytic mechanism of the tau class glutathione transferase from Glycine max (2010), Biochim. Biophys. Acta, 1804, 662-667.
    View publication on PubMed
Search for more literature for 2.5.1.18

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of wild-type and mutannt isozymes GSTU4-4 Glycine max

Protein Variants

Protein Variants Comment Organism
N48A site-directed mutagensiss of a strictly conserved residue, the mutation significantly affects substrate binding and specificity. Mutation of Asn48 and Pro49 residues may bring about secondary effects altering the thermal stability and the catalytic activity (kcat) of the enzyme without affecting the nature of the rate-limiting step of the catalytic reaction Glycine max
P49A site-directed mutagensiss of a strictly conserved residue, the mutation significantly affects substrate binding and specificity. Mutation of Asn48 and Pro49 residues may bring about secondary effects altering the thermal stability and the catalytic activity (kcat) of the enzyme without affecting the nature of the rate-limiting step of the catalytic reaction Glycine max
S13A site-directed mutagensiss of a strictly conserved residue, the mutation significantly affects substrate binding and specificity Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information steady-state kinetic analysis of wild-type and mutannt isozymes GSTU4-4, overview Glycine max
0.14
-
 1-chloro-2,4-dinitrobenzene pH 6.5, 30°C, recombinant GSTU4-4 mutant P49A Glycine max
0.158
-
 1-chloro-2,4-dinitrobenzene pH 6.5, 30°C, recombinant wild-type GSTU4-4 Glycine max
0.159
-
 glutathione pH 6.5, 30°C, recombinant wild-type GSTU4-4 Glycine max
0.26
-
 1-chloro-2,4-dinitrobenzene pH 6.5, 30°C, recombinant GSTU4-4 mutant D48A Glycine max
0.29
-
 glutathione pH 6.5, 30°C, recombinant GSTU4-4 mutant D48A Glycine max
0.35
-
 glutathione pH 6.5, 30°C, recombinant GSTU4-4 mutant S13A Glycine max
0.47
-
 1-chloro-2,4-dinitrobenzene pH 6.5, 30°C, recombinant GSTU4-4 mutant S13A Glycine max
0.5
-
 glutathione pH 6.5, 30°C, recombinant GSTU4-4 mutant P49A Glycine max

Organism

Organism UniProt Comment Textmining
Glycine max O49235 isozyme GSTU4-4
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutannt isozymes GSTU4-4 by glutathione affinity chromatography Glycine max

Reaction

Reaction Comment Organism Reaction ID
RX + glutathione = HX + R-S-glutathione catalytic mechanism, ovverview Glycine max
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-chloro-2,4-dinitrobenzene + glutathione residue Ser13 is directly involved in the reaction chemistry and the correct positioning of GSH and CDNB in the ternary catalytic complex. Asn48 and Pro49 have a direct role on the structural integrity of the GSH-binding site (G-site) Glycine max 2,4-dinitrophenyl-glutathione + HCl
-
 ?

Subunits

Subunits Comment Organism
More secondary structure of GmGSTU4-4 from PDB ID 2VO4 and sequence comparisons, overview Glycine max

Synonyms

Synonyms Comment Organism
GmGSTU4-4
-
 Glycine max
tau class glutathione transferase
-
 Glycine max
tau class GSTU4-4
-
 Glycine max

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Glycine max

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 purified recommbinant wild-type and mutant isozymes GSTU4-4, 100 mM potassium phosphate, pH 7, stable up to Glycine max
57.1
-
 Tm for the Pro49Ala mutant Glycine max
60.7
-
 Tm for the Asn48Ala mutant Glycine max
64.8
-
 Tm for the Ser13Ala mutant Glycine max
68.4
-
 Tm for the wild-type isozyme GSTU4-4 Glycine max

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0897
-
 1-chloro-2,4-dinitrobenzene pH 6.5, 30°C, recombinant GSTU4-4 mutant S13A Glycine max
1.45
-
 1-chloro-2,4-dinitrobenzene pH 6.5, 30°C, recombinant GSTU4-4 mutant D48A Glycine max
2.48
-
 1-chloro-2,4-dinitrobenzene pH 6.5, 30°C, recombinant wild-type GSTU4-4 Glycine max
5.9
-
 1-chloro-2,4-dinitrobenzene pH 6.5, 30°C, recombinant GSTU4-4 mutant P49A Glycine max

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 assay at Glycine max

pH Range

pH Minimum pH Maximum Comment Organism
5.2 8.5 activity range, profile overview Glycine max

General Information

General Information Comment Organism
additional information role of conserved residues Ser13, Asn48 and Pro49 in the catalytic mechanism of the tau class glutathione transferase, overview Glycine max

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.19
-
 1-chloro-2,4-dinitrobenzene pH 6.5, 30°C, recombinant GSTU4-4 mutant S13A Glycine max
5.58
-
 1-chloro-2,4-dinitrobenzene pH 6.5, 30°C, recombinant GSTU4-4 mutant D48A Glycine max
15.7
-
 1-chloro-2,4-dinitrobenzene pH 6.5, 30°C, recombinant wild-type GSTU4-4 Glycine max
42.1
-
 1-chloro-2,4-dinitrobenzene pH 6.5, 30°C, recombinant GSTU4-4 mutant P49A Glycine max