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Literature summary for 2.5.1.18 extracted from

  • Norrgard, M.A.; Hellman, U.; Mannervik, B.
    Cys-X scanning for expansion of active-site residues and modulation of catalytic functions in a glutathione transferase (2011), J. Biol. Chem., 286, 16871-16878.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.5.1.18

Protein Variants

Protein Variants Comment Organism
C87A/C115A/C174A/M212C site-directed mutagensis of isozyme GST M2-2, mutation of the catalytic site residue M212 and of all cysteines, the mutant shows altered substrate specificity compared to the wild-type isozyme. C87A/C115A/C174A/M212C, which serves as the parental enzyme for the alkylated variants, is more active than mutant C87A/C115A/C174A with 1-chloro-2,4-dinitrobenzene, cyanoDMNG, and aminochrome but shows only half of the NPG activity. Effects of alkylation of the C87A/C115A/C174A/M212C mutant on the substrate selectivity profile, overview Homo sapiens
M212C site-directed mutagensis of isozyme GST M2-2, mutation of the catalytic site residue M212. Additionall chemical modifications at mutant Cys212, e.g. by attachment of alkyl chains, alter the substrate specificity and activity of the mutant enzyme with alternative substrates, effects of alkylation of the C87A/C115A/C174A/M212C mutant on the substrate selectivity profile, overview Homo sapiens
additional information azathioprine activity of wild-type hGST M2-2 can be enhanced 30fold by two point mutations of other residues in the active site Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-chloro-2,4-dinitrobenzene + glutathione aromatic substitution Homo sapiens S-(2,4-dinitrophenyl)glutathione + HCl
-
 ?
aminochrome + glutathione
-
 Homo sapiens ?
-
 ?
azathioprine + glutathione aromatic substitution. Wild-type hGST M2-2 has modest activity with azathioprine, but mutant C87A/C115A/C174A/M212C displays no detectable activity. The azathioprine activity of wild-type hGST M2-2 can be enhanced 30fold by two point mutations of other residues in the active site Homo sapiens ?
-
 ?
[(methylamino)[methyl(nitroso)amino]methyl]cyanamide + glutathione transnitrosylation Homo sapiens [bis(methylamino)methyl]cyanamide + S-nitrosoglutathione
-
 ?
[3-(4-nitrophenyl)oxiran-2-yl]methanol + glutathione
-
 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
glutathione transferase M2-2
-
 Homo sapiens
GST M2-2
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
 assay at Homo sapiens

General Information

General Information Comment Organism
additional information role for mutant Cys in mutant M212C in the transnitrosylation reaction with cyanoDMNG, while the Cys residue is not required in the aromatic substitution with 1-chloro-2,4-dinitrobenzene, for epoxide opening with NPG, and for addition to the ortho-quinone with aminochrome Homo sapiens