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Literature summary for 2.5.1.18 extracted from

  • Yin, L.; Song, J.; Board, P.G.; Yu, Y.; Han, X.; Wei, J.
    Characterization of selenium-containing glutathione transferase zeta1-1 with high GPX activity prepared in eukaryotic cells (2013), J. Mol. Recognit., 26, 38-45.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
transient expression of His6-tagged wild-type hGSTZ1-1 and mutant seleno-hGSTZ1-1 in HEK-293T cells, expression of GSTZ1-1 in Escherichia coli strain M15 Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information site-directed mutagenesis and the inclusion of a selenocysteine insertion sequence to engineer the expression in eukaryotic cells of human glutathione transferase zeta1-1 with selenocysteine in the active site. This modification converts hGSTZ1-1 into an active glutathione peroxidase, EC 1.11.1.9, while the wild-type GSTZ1-1 shows no glutathione peroxidase at all, Michaelis-Menten kinetics and ping-pong mechanism of mutant Se-GSTZ1-1 with H2O2 and GSH, overview. Selenocysteine at the active site plays an important role in the determination of catalytic specificity and performance, the ping-pong catalytic mechanism of Se-hGSTZ1-1 is similar to that of the natural glutathione peroxidase Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24300
-
x * 24300, recombinant His6-tagged wild-type GSTZ1-1, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged wild-type hGSTZ1-1 and mutant seleno-hGSTZ1-1 from HEK-293T cells by nickel affinity chromatography Homo sapiens

Subunits

Subunits Comment Organism
? x * 24300, recombinant His6-tagged wild-type GSTZ1-1, SDS-PAGE Homo sapiens
More wild-type hGSTZ1-1 and mutant seleno-hGSTZ1-1 three-dimensional structure modeling, overview Homo sapiens

Synonyms

Synonyms Comment Organism
selenium-containing glutathione transferase zeta1-1
-
Homo sapiens
seleno-hGSTZ1-1
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Homo sapiens