Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-phosphonomethylglycine | 0.006-0.008 mM, 50% inhibition, 1 mM, complete inhibition | Zea mays |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.028 | - |
phosphoenolpyruvate | isoenzyme II | Zea mays | |
0.029 | - |
shikimate 3-phosphate | isoenzyme II | Zea mays | |
0.033 | - |
phosphoenolpyruvate | isoenzyme I | Zea mays | |
0.033 | - |
shikimate 3-phosphate | isoenzyme I | Zea mays |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | isoenzymes I and II | Zea mays | 9507 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53600 | - |
1 * 53600, isoenzyme II, SDS-PAGE | Zea mays |
54900 | - |
1 * 54900, isoenzyme I, SDS-PAGE | Zea mays |
58100 | - |
isoenzyme II, gel filtration | Zea mays |
58700 | - |
isoenzyme I, gel filtration | Zea mays |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
no activity in mammals | - |
- |
- |
Petunia x hybrida | - |
- |
- |
Zea mays | - |
var. Black Mexican Sweet, isoenzymes I and II | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, DEAE-Sephacel, cellulose phosphate column, isoenzymes I and II | Zea mays |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
61 | - |
isoenzyme I | Zea mays |
94.7 | - |
isoenzyme II | Zea mays |
Storage Stability | Organism |
---|---|
-20°C, 3 months, 15% loss of activity | Zea mays |
0°C, 30 d, 25% loss of activity | Zea mays |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + 3-phosphoshikimate | - |
Zea mays | phosphate + 5-enolpyruvylshikimate 3-phosphate | i.e. 5-O-(1-carboxyvinyl)-3-phosphoshikimate | r | |
phosphoenolpyruvate + 3-phosphoshikimate | - |
Petunia x hybrida | phosphate + 5-enolpyruvylshikimate 3-phosphate | i.e. 5-O-(1-carboxyvinyl)-3-phosphoshikimate | r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 53600, isoenzyme II, SDS-PAGE | Zea mays |
monomer | 1 * 54900, isoenzyme I, SDS-PAGE | Zea mays |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
isoenzymes I and II | Zea mays |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
isoenzyme I, 50% loss of activity after 20 min, isoenzyme II, 50% loss of activity after 60 min | Zea mays |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | 7.5 | isoenzymes I and II | Zea mays |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00012 | 0.00013 | N-phosphonomethylglycine | isoenzymes I and II, vs. phosphoenolpyruvate | Zea mays | |
0.0094 | - |
N-phosphonomethylglycine | isoenzyme I, vs. shikimate 3-phosphate | Zea mays | |
0.012 | - |
N-phosphonomethylglycine | isoenzyme II, vs. shikimate 3-phosphate | Zea mays |