Application | Comment | Organism |
---|---|---|
agriculture | transgenic Arabidopsis thaliana plants expressing the enzyme from Rahnella aquatilis are more resistant to glyphosate exposure than plants expressing the Escherichia coli enzyme | Rahnella aquatilis |
Protein Variants | Comment | Organism |
---|---|---|
G96A | mutant with improved resistance towards glyphosate | Rahnella aquatilis |
T38K/R40V/R222Q/S224V/I225V/Q226K | mutant with the c-strand of subdomain 3 and the f-strand of subdomain 5 substituted by the corresponding region of the Escherichia coli enzyme. The mutant enzyme exhibits greater sensitivity to glyphosate than the wild type with little change of affinity for substrates shikimate-3-phosphate and phosphoenolpyruvate | Rahnella aquatilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
glyphosate | - |
Rahnella aquatilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.078 | - |
phosphoenolpyruvate | wild-type, pH 7.0, 28°C | Rahnella aquatilis | |
0.084 | - |
3-phosphoshikimate | mutant G96A, pH 7.0, 28°C | Rahnella aquatilis | |
0.086 | - |
3-phosphoshikimate | wild-type, pH 7.0, 28°C | Rahnella aquatilis | |
0.148 | - |
phosphoenolpyruvate | mutant G96A, pH 7.0, 28°C | Rahnella aquatilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
x * 47000, SDS-PAGE | Rahnella aquatilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rahnella aquatilis | C9EGX2 | - |
- |
Rahnella aquatilis GR20 | C9EGX2 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + 3-phosphoshikimate | - |
Rahnella aquatilis | phosphate + 5-enolpyruvylshikimate 3-phosphate | - |
? | |
phosphoenolpyruvate + 3-phosphoshikimate | - |
Rahnella aquatilis GR20 | phosphate + 5-enolpyruvylshikimate 3-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 47000, SDS-PAGE | Rahnella aquatilis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.049 | - |
glyphosate | wild-type, pH 7.0, 28°C | Rahnella aquatilis | |
0.45 | - |
glyphosate | mutant G96A, pH 7.0, 28°C | Rahnella aquatilis |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.26 | - |
wild-type, pH 7.0, 28°C | Rahnella aquatilis | glyphosate | |
0.34 | - |
mutant G96A, pH 7.0, 28°C | Rahnella aquatilis | glyphosate |
General Information | Comment | Organism |
---|---|---|
physiological function | enzyme is able to restore the growth of the Escherichia coli mutant ER2799, with the 5-enolpyruvylshikimate-3-phosphate synthase gene deleted from its genome, on an M9 minimal medium containing 60 mM glyphosate | Rahnella aquatilis |