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Literature summary for 2.5.1.19 extracted from

  • Peng, R.H.; Tian, Y.S.; Xiong, A.S.; Zhao, W.; Fu, X.Y.; Han, H.J.; Chen, C.; Jin, X.F.; Yao, Q.H.
    A novel 5-enolpyruvylshikimate-3-phosphate synthase from Rahnella aquatilis with significantly reduced glyphosate sensitivity (2012), PLoS ONE, 7, e39579.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
agriculture transgenic Arabidopsis thaliana plants expressing the enzyme from Rahnella aquatilis are more resistant to glyphosate exposure than plants expressing the Escherichia coli enzyme Rahnella aquatilis

Protein Variants

Protein Variants Comment Organism
G96A mutant with improved resistance towards glyphosate Rahnella aquatilis
T38K/R40V/R222Q/S224V/I225V/Q226K mutant with the c-strand of subdomain 3 and the f-strand of subdomain 5 substituted by the corresponding region of the Escherichia coli enzyme. The mutant enzyme exhibits greater sensitivity to glyphosate than the wild type with little change of affinity for substrates shikimate-3-phosphate and phosphoenolpyruvate Rahnella aquatilis

Inhibitors

Inhibitors Comment Organism Structure
glyphosate
-
Rahnella aquatilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.078
-
phosphoenolpyruvate wild-type, pH 7.0, 28°C Rahnella aquatilis
0.084
-
3-phosphoshikimate mutant G96A, pH 7.0, 28°C Rahnella aquatilis
0.086
-
3-phosphoshikimate wild-type, pH 7.0, 28°C Rahnella aquatilis
0.148
-
phosphoenolpyruvate mutant G96A, pH 7.0, 28°C Rahnella aquatilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
x * 47000, SDS-PAGE Rahnella aquatilis

Organism

Organism UniProt Comment Textmining
Rahnella aquatilis C9EGX2
-
-
Rahnella aquatilis GR20 C9EGX2
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + 3-phosphoshikimate
-
Rahnella aquatilis phosphate + 5-enolpyruvylshikimate 3-phosphate
-
?
phosphoenolpyruvate + 3-phosphoshikimate
-
Rahnella aquatilis GR20 phosphate + 5-enolpyruvylshikimate 3-phosphate
-
?

Subunits

Subunits Comment Organism
? x * 47000, SDS-PAGE Rahnella aquatilis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.049
-
glyphosate wild-type, pH 7.0, 28°C Rahnella aquatilis
0.45
-
glyphosate mutant G96A, pH 7.0, 28°C Rahnella aquatilis

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.26
-
wild-type, pH 7.0, 28°C Rahnella aquatilis glyphosate
0.34
-
mutant G96A, pH 7.0, 28°C Rahnella aquatilis glyphosate

General Information

General Information Comment Organism
physiological function enzyme is able to restore the growth of the Escherichia coli mutant ER2799, with the 5-enolpyruvylshikimate-3-phosphate synthase gene deleted from its genome, on an M9 minimal medium containing 60 mM glyphosate Rahnella aquatilis