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Literature summary for 2.5.1.19 extracted from

  • Tan, X.L.; Othman, R.Y.; Teo, C.H.
    Isolation and functional characterization of 5-enolpyruvylshikimate 3-phosphate synthase gene from glyphosate-tolerant Pseudomonas nitroreducens strains FY43 and FY47 (2020), 3 Biotech, 10, 183 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.5.1.19

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Pseudomonas nitroreducens

Protein Variants

Protein Variants Comment Organism
A242V the mutation leads to sensitivity to 8.768% glyphosate Pseudomonas nitroreducens
A329T the mutation leads to sensitivity to 8.768% glyphosate Pseudomonas nitroreducens
G292C the mutation leads to sensitivity to 8.768% glyphosate Pseudomonas nitroreducens
G292S the mutation does not affect sensitivity to glyphosate Pseudomonas nitroreducens
L184F the mutation leads to sensitivity to 8.768% glyphosate Pseudomonas nitroreducens
L35F the mutation leads to sensitivity to 8.768% glyphosate Pseudomonas nitroreducens
N265S the mutation leads to sensitivity to 8.768% glyphosate Pseudomonas nitroreducens
P71L the mutation leads to sensitivity to 8.768% glyphosate Pseudomonas nitroreducens
R21C the mutation leads to sensitivity to 8.768% glyphosate Pseudomonas nitroreducens
T258A the mutation leads to sensitivity to 8.768% glyphosate Pseudomonas nitroreducens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphoenolpyruvate + 3-phosphoshikimate Pseudomonas nitroreducens
-
 phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate
-
 ?
phosphoenolpyruvate + 3-phosphoshikimate Pseudomonas nitroreducens FY43
-
 phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate
-
 ?
phosphoenolpyruvate + 3-phosphoshikimate Pseudomonas nitroreducens FY47
-
 phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas nitroreducens
-
-
-
Pseudomonas nitroreducens FY43
-
-
-
Pseudomonas nitroreducens FY47
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + 3-phosphoshikimate
-
 Pseudomonas nitroreducens phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate
-
 ?
phosphoenolpyruvate + 3-phosphoshikimate
-
 Pseudomonas nitroreducens FY43 phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate
-
 ?
phosphoenolpyruvate + 3-phosphoshikimate
-
 Pseudomonas nitroreducens FY47 phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate
-
 ?

Synonyms

Synonyms Comment Organism
3-phosphoshikimate 1-carboxyvinyl-transferase
-
 Pseudomonas nitroreducens
5-enolpyruvylshikimate 3-phosphate synthase
-
 Pseudomonas nitroreducens
EPSPS
-
 Pseudomonas nitroreducens