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Literature summary for 2.5.1.2 extracted from

  • Bos, M.; Kozik, A.
    Some molecular and enzymatic properties of a homogeneous preparation of thiaminase I purified from carp liver (2000), J. Protein Chem., 19, 75-84.
    View publication on PubMed

Localization

Localization Comment Organism GeneOntology No. Textmining
cell associated
-
Cyprinus carpio
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
gel filtration Cyprinus carpio

Organism

Organism UniProt Comment Textmining
Cyprinus carpio
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by ammonium precipitation, CM-cellulose column, DEAE-cellulose column, Cibacron blue Sepharose column, Sephacryl S-300 column, Superdex-200 and Superdex-75 column Cyprinus carpio

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Cyprinus carpio
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1'-methylthiamine + aniline 22% of activity with thiamine Cyprinus carpio 4-methyl-5-(2-hydroxyethyl)-thiazole + 4-amino-5-(anilinomethyl)-1,2-dimethylpyrimidin-1-ium
-
?
2'-ethylthiamine + aniline 63% of activity with thiamine Cyprinus carpio 4-methyl-5-(2-hydroxyethyl)-thiazole + 5-(anilinomethyl)-2-ethylpyrimidin-4-amine
-
?
2-(1-hydroxyethyl)thiamine + aniline 92% of activity with thiamine Cyprinus carpio 4-methyl-2-(1-hydroxyethyl)-5-(2-hydroxyethyl)-thiazole + 5-(anilinomethyl)-2-methylpyrimidin-4-amine
-
?
5-chloroethylthiamine + aniline 67% of activity with thiamine Cyprinus carpio 4-methyl-5-chloroethyl-thiazole + 5-(anilinomethyl)-2-methylpyrimidin-4-amine
-
?
5-nor-thiamine + aniline 88% of activity with thiamine Cyprinus carpio 4-methyl-thiazole + 5-(anilinomethyl)-2-methylpyrimidin-4-amine
-
?
additional information no activity with 4'-deaminothiamine and 4'-oxythiamine suggest that 4'-amino group is essential for enzyme activity Cyprinus carpio ?
-
?
O-(beta-D-galactosyl)thiamine + aniline 63% of activity with thiamine Cyprinus carpio 4-methyl-5-(2-hydroxyethyl-O-beta-D-galactosyl)-thiazole + 5-(anilinomethyl)-2-methylpyrimidin-4-amine
-
?
O-benzoylthiamine + aniline 91% of activity with thiamine Cyprinus carpio 2-(4-methyl-1,3-thiazol-5-yl)ethyl benzoate + 5-(anilinomethyl)-2-methylpyrimidin-4-amine
-
?
O-S-diacetylthiamine + aniline 50% of activity with thiamine Cyprinus carpio 1-acetyl-4-methyl-5-(2-hydroxyethyl-O-acetyl)-thiazole + 5-(anilinomethyl)-2-methylpyrimidin-4-amine
-
?
O-succinylthiamine + aniline 83% of activity with thiamine Cyprinus carpio 4-methyl-5-(2-hydroxyethyl-O-succinyl)-thiazole + 5-(anilinomethyl)-2-methylpyrimidin-4-amine
-
?
pyrithiamine + aniline 72% of activity with thiamine Cyprinus carpio 2-(2-methylpyridin-3-yl)ethanol + 5-(anilinomethyl)-2-methylpyrimidin-4-amine
-
?
tetrahydrothiamine + aniline 84% of activity with thiamine Cyprinus carpio ?
-
?
thiamine + 2-mercaptoethanol 63% of activity with aniline Cyprinus carpio ?
-
?
thiamine + aniline taken as 100% of activity of purified enzyme Cyprinus carpio 5-(anilinomethyl)-2-methylpyrimidin-4-amine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
?
thiamine + imidazole 24% of activity with aniline Cyprinus carpio 4-methyl-5-(2-hydroxyethyl)-thiazole + 5-(1H-imidazol-1-yl-methyl)-2-methylpyrimidin-4-amine
-
?
thiamine + L-cysteine 50% of activity with aniline Cyprinus carpio 4-methyl-5-(2-hydroxyethyl)-thiazole + 2-[[(4-amino-2-ethylpyrimidin-5-yl)methyl]amino]-3-mercaptopropanoic acid
-
?
thiamine + pyridine best substrate with aniline Cyprinus carpio heteropyrithiamine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
?
thiamine disulfide + aniline 26% of activity with thiamine Cyprinus carpio ?
-
?
thiamine phosphate + aniline 64% of activity with thiamine Cyprinus carpio 2-(4-methyl-1,3-thiazol-5-yl)ethyl dihydrogenphosphate + 5-(anilinomethyl)-2-methylpyrimidin-4-amine
-
?
thiothiamine + aniline 12% of activity with thiamine Cyprinus carpio 4-methyl-5-(2-hydroxyethyl)-2-thio-thiazole + 5-(anilinomethyl)-2-methylpyrimidin-4-amine
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Cyprinus carpio

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7
-
Cyprinus carpio