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Literature summary for 2.5.1.20 extracted from

  • Archer, B.L.; Cockbain, E.G.
    Rubber transferase from hevea brasiliensis latex (1969), Methods Enzymol., 15, 476-480.
No PubMed abstract available
Search for more literature for 2.5.1.20

Activating Compound

Activating Compound Comment Organism Structure
neryl diphosphate accelerates activity Hevea brasiliensis
sulfhydryl compound essential for full activity Hevea brasiliensis

Inhibitors

Inhibitors Comment Organism Structure
EDTA 50 mM, strong inhibition Hevea brasiliensis
farnesyl diphosphate
-
 Hevea brasiliensis
iodoacetamide
-
 Hevea brasiliensis
N-ethylmaleimide
-
 Hevea brasiliensis
p-chloromercuribenzoate 50 mM, strong inhibition Hevea brasiliensis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ magnesium compounds essential for full activity, cannot be replaced by manganese, cobalt or iron Hevea brasiliensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 gel filtration Hevea brasiliensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
isopentenyl diphosphate + cis-1,4-polyisoprene Hevea brasiliensis incorporation of isopentenyl diphosphate into rubber diphosphate + a poly-cis-polyprenyl diphosphate longer by one C5 unit
-
 ?

Organism

Organism UniProt Comment Textmining
Hevea brasiliensis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
pH 5.4, ammonium sulfate, Sephadex G-100, DEAE-Sephadex, partial purification Hevea brasiliensis

Source Tissue

Source Tissue Comment Organism Textmining
latex
-
 Hevea brasiliensis
-
leaf
-
 Hevea brasiliensis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isopentenyl diphosphate + cis-1,4-polyisoprene strictly stereospecific, yields exclusively cis-polyisoprene from isopentenyl diphosphate Hevea brasiliensis diphosphate + a poly-cis-polyprenyl diphosphate longer by one C5 unit
-
 ?
isopentenyl diphosphate + cis-1,4-polyisoprene of rubber particles Hevea brasiliensis diphosphate + a poly-cis-polyprenyl diphosphate longer by one C5 unit
-
 ?
isopentenyl diphosphate + cis-1,4-polyisoprene incorporation of isopentenyl diphosphate into rubber Hevea brasiliensis diphosphate + a poly-cis-polyprenyl diphosphate longer by one C5 unit
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Hevea brasiliensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 10 min, partial denaturation Hevea brasiliensis
60
-
 10 min, complete inactivation Hevea brasiliensis

Cofactor

Cofactor Comment Organism Structure
additional information ascorbic acid is not cofactor Hevea brasiliensis