Literature summary for 2.5.1.20 extracted from

Rojruthai, P.; Sakdapipanich, J.T.; Takahashi, S.; Hyegin, L.; Noike, M.; Koyama, T.; Tanaka, Y.
In vitro synthesis of high molecular weight rubber by Hevea small rubber particles (2010), J. Biosci. Bioeng., 109, 107-114.

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
isopentenyl diphosphate + cis-1,4-polyisoprene	Hevea brasiliensis	50 mM phosphate buffer, pH 7.5, 40 mM 2-mercaptoethanol, 40 mM KF, 8 mM MgCl2 + substrates, 30°C, 70% incorporation small rubber particles, 50% incorporation with FL-latex, 13% with washed small particles, 4% with highly purified washed small particles, with farnesyl diphosphate or dimethylallyl diphosphate as allylic initiators 70% incorporation, similar as for small rubber particles without allylic initiators	diphosphate + a poly-cis-polyprenyl diphosphate longer by one C5 unit	-	?

Organism

Organism	UniProt	Comment	Textmining
Hevea brasiliensis	-	rubber tree clone RRIM 600	-

Purification (Commentary)

Purification (Comment)	Organism
centrifugation to separate small rubber particles from the lower layer, dilution with 0.01 M phosphate buffer, pH 7.0 to 30% dry rubber content, washed particles are produced by washing with 0.25 M Tris-HCl, pH 8.0, with 1% Tween-20 and 5 mM 2-mercaptoethanol, applied to a Ultrogel AcA-44 column, centrifuged and diluted to 15% dry rubber content, highly purified small particles are produced by further centrifugation with 0.5% Triton X-100, exchange by buffer and centrifugation, cream phase diluted to 15% dry rubber content	Hevea brasiliensis

Purification (Comment)

Organism

centrifugation to separate small rubber particles from the lower layer, dilution with 0.01 M phosphate buffer, pH 7.0 to 30% dry rubber content, washed particles are produced by washing with 0.25 M Tris-HCl, pH 8.0, with 1% Tween-20 and 5 mM 2-mercaptoethanol, applied to a Ultrogel AcA-44 column, centrifuged and diluted to 15% dry rubber content, highly purified small particles are produced by further centrifugation with 0.5% Triton X-100, exchange by buffer and centrifugation, cream phase diluted to 15% dry rubber content

Hevea brasiliensis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	2.3 microM isopentenyl diphosphate incorporation/g dry rubber/4 h for small rubber particles, 0.7 microM isopentenyl diphosphate incorporation/g dry rubber/4 h for purified and washed small rubber particles, almost no isopentenyl diphosphate incorporation/g dry rubber/4 h for washed and highly purified small rubber particles, in vitro, 50 mM phosphate buffer, pH 7.5, 40 mM 2-mercaptoethanol, 40 mM KF, 8 mM MgCl2 + substrates, 30°C, small rubber particle activity decreases with increasing dimethylallyl diphosphate concentration, dimethylallyl diphosphate slightly increases activity in washed small particles not in a dose-dependent manner	Hevea brasiliensis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
isopentenyl diphosphate + cis-1,4-polyisoprene	50 mM phosphate buffer, pH 7.5, 40 mM 2-mercaptoethanol, 40 mM KF, 8 mM MgCl2 + substrates, 30°C, 70% incorporation small rubber particles, 50% incorporation with FL-latex, 13% with washed small particles, 4% with highly purified washed small particles, with farnesyl diphosphate or dimethylallyl diphosphate as allylic initiators 70% incorporation, similar as for small rubber particles without allylic initiators	Hevea brasiliensis	diphosphate + a poly-cis-polyprenyl diphosphate longer by one C5 unit	-	?

Synonyms

Synonyms	Comment	Organism
cis-prenyltransferase	-	Hevea brasiliensis
rubber transferase	-	Hevea brasiliensis