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Literature summary for 2.5.1.21 extracted from

  • Kroon, P.A.; Threlfall, D.R.
    Squalene synthase from cell suspension cultures of Tabernaemontana divaricata (1997), Phytochemistry, 45, 1157-1163.
No PubMed abstract available
Search for more literature for 2.5.1.21

Inhibitors

Inhibitors Comment Organism Structure
Mg2+ above 35 mM Tabernaemontana divaricata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0078
-
 farnesyl diphosphate
-
 Tabernaemontana divaricata
0.045
-
 NADPH
-
 Tabernaemontana divaricata

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Tabernaemontana divaricata 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required, best at 10 mM, inhibition above 35 mM Tabernaemontana divaricata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000 60000 gel filtration Tabernaemontana divaricata
64000
-
 1 * 64000, SDS-PAGE Tabernaemontana divaricata

Organism

Organism UniProt Comment Textmining
Tabernaemontana divaricata
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Tabernaemontana divaricata

Reaction

Reaction Comment Organism Reaction ID
2 (2E,6E)-farnesyl diphosphate + NAD(P)H + H+ = squalene + 2 diphosphate + NAD(P)+ enzyme also catalyses the reduction of presqualene diphosphate by NADPH to squalene Tabernaemontana divaricata
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate + NADPH + H+
-
 Tabernaemontana divaricata squalene + diphosphate + NADP+
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 64000, SDS-PAGE Tabernaemontana divaricata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8
-
 Tabernaemontana divaricata