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Literature summary for 2.5.1.21 extracted from
- Cloning, solubilization, and characterization of squalene synthase from Thermosynechococcus elongatus BP-1 (2008), J. Bacteriol., 190, 3808-3816.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | maximal activity at 2 mM | Thermosynechococcus vestitus |  |
| Tween 80 | maximal activity at 2% v/v | Thermosynechococcus vestitus | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli, His-tagged protein found exclusively in inclusion bodies | Thermosynechococcus vestitus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| zaragozic acid A | pH 7.2, 37°C | Thermosynechococcus vestitus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00097 | - |
farnesyl diphosphate | pH 7.2, 37°C | Thermosynechococcus vestitus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Thermosynechococcus vestitus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 39900 | - |
x * 42000, SDS-PAGE, x * 41886, electrospray mass spectrometry, x * 39900, calculated | Thermosynechococcus vestitus |
| 41886 | - |
x * 42000, SDS-PAGE, x * 41886, electrospray mass spectrometry, x * 39900, calculated | Thermosynechococcus vestitus |
| 42000 | - |
x * 42000, SDS-PAGE, x * 41886, electrospray mass spectrometry, x * 39900, calculated | Thermosynechococcus vestitus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermosynechococcus vestitus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged protein found exclusively in inclusion bodies. Solubilization in presence of glycerol | Thermosynechococcus vestitus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, stable for at least 6 months | Thermosynechococcus vestitus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 farnesyl diphosphate | - |
Thermosynechococcus vestitus | presqualene diphosphate + diphosphate | in absence of NADPH, formation of presqualene diphosphate | ? | |
| farnesyl diphosphate + NADPH + H+ | - |
Thermosynechococcus vestitus | squalene + diphosphate + NADP+ | sole product | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 42000, SDS-PAGE, x * 41886, electrospray mass spectrometry, x * 39900, calculated | Thermosynechococcus vestitus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Thermosynechococcus vestitus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.74 | - |
farnesyl diphosphate | - |
Thermosynechococcus vestitus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
- |
Thermosynechococcus vestitus |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.0000955 | - |
pH 7.2, 37°C | Thermosynechococcus vestitus | zaragozic acid A | |
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