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Literature summary for 2.5.1.27 extracted from
- Molecular cloning, expression, and characterization of adenylate isopentenyltransferase from hop (Humulus lupulus L.) (2004), Phytochemistry, 65, 2439-2446.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Humulus lupulus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0162 | - |
ATP | pH 8.0, 25°C | Humulus lupulus |  |
| 0.0193 | - |
ADP | pH 8.0, 25°C | Humulus lupulus | |
| 0.0195 | - |
dimethylallyl diphosphate | pH 8.0, 25°C | Humulus lupulus | |
| 0.759 | - |
AMP | pH 8.0, 25°C | Humulus lupulus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | dependent on | Humulus lupulus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37000 | - |
x * 37000, SDS-PAGE | Humulus lupulus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Humulus lupulus | Q5GHF7 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Humulus lupulus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cone | - |
Humulus lupulus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dimethylallyl diphosphate + ADP | the enzyme prefers ADP and ATP rather than AMP | Humulus lupulus | ? | - |
? | |
| dimethylallyl diphosphate + AMP | the enzyme prefers ADP and ATP rather than AMP | Humulus lupulus | diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate | - |
? | |
| dimethylallyl diphosphate + ATP | the enzyme prefers ADP and ATP rather than AMP | Humulus lupulus | ? | - |
? | |
| additional information | no activity with GMP, IMP, CMP and UMP | Humulus lupulus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 37000, SDS-PAGE | Humulus lupulus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AIPT | - |
Humulus lupulus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00828 | - |
AMP | pH 8.0, 25°C | Humulus lupulus | |
| 0.022 | - |
ATP | pH 8.0, 25°C | Humulus lupulus | |
| 0.024 | - |
ADP | pH 8.0, 25°C | Humulus lupulus | |
| 0.027 | - |
dimethylallyl diphosphate | pH 8.0, 25°C | Humulus lupulus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | either in Tris-HCl or potassium-phosphate buffer | Humulus lupulus |
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