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Literature summary for 2.5.1.27 extracted from

  • Abe, I.; Tanaka, H.; Abe, T.; Noguchi, H.
    Enzymatic formation of unnatural cytokinin analogs by adenylate isopentenyltransferase from mulberry (2007), Biochem. Biophys. Res. Commun., 355, 795-800.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Morus alba

Protein Variants

Protein Variants Comment Organism
D49A almost complete loss of activity compared to the wild type enzyme Morus alba
F120A almost complete loss of activity compared to the wild type enzyme Morus alba
F157A almost complete loss of activity compared to the wild type enzyme Morus alba
F93A almost complete loss of activity compared to the wild type enzyme Morus alba
Q255A almost complete loss of activity compared to the wild type enzyme Morus alba
W159A almost complete loss of activity compared to the wild type enzyme Morus alba
Y153A almost complete loss of activity compared to the wild type enzyme Morus alba
Y170A retains 84% activity compared to the wild type enzyme Morus alba
Y217A almost complete loss of activity compared to the wild type enzyme Morus alba
Y54A almost complete loss of activity compared to the wild type enzyme Morus alba

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0374
-
dimethylallyl diphosphate
-
Morus alba
0.0682
-
ADP
-
Morus alba

Metals/Ions

Metals/Ions Comment Organism Structure
MgCl2 dependent on presence of divalent cation, addition of 2 mM MnCl2 is 1.3 times more effective than that of 10 mM MgCl2 Morus alba
MnCl2 dependent on presence of divalent cation, addition of 2 mM MnCl2 is 1.3 times more effective than that of 10 mM MgCl2 Morus alba

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35810
-
calculated from sequence of cDNA Morus alba

Organism

Organism UniProt Comment Textmining
Morus alba Q08ET4 mulberry
-

Purification (Commentary)

Purification (Comment) Organism
Ni-chelate affinity column chromatography Morus alba

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Morus alba
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-hydroxy-3-methyl-2-(E)-butenyl diphosphate + ADP 4-hydroxy-3-methyl-2-(E)-butenyl diphosphate is a very poor substrate Morus alba diphosphate + N6-(4-hydroxy-3-methyl-2-(E)-butenyl)ADP
-
?
4-hydroxy-3-methyl-2-(E)-butenyl diphosphate + ATP 4-hydroxy-3-methyl-2-(E)-butenyl diphosphate is a very poor substrate Morus alba trans-zeatin riboside 5'-triphosphate + diphosphate
-
?
dimethylallyl diphosphate + ADP
-
Morus alba diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
-
ir
dimethylallyl diphosphate + AMP AMP is a poor substrate Morus alba diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate
-
ir
dimethylallyl diphosphate + ATP
-
Morus alba diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
-
ir
dimethylallyl diphosphate + CDP
-
Morus alba ?
-
ir
dimethylallyl diphosphate + dADP
-
Morus alba diphosphate + N6-(dimethylallyl)dADP
-
ir
dimethylallyl diphosphate + dATP
-
Morus alba diphosphate + N6-(dimethylallyl)dATP
-
ir
dimethylallyl diphosphate + GDP GDP is a poor substrate Morus alba ?
-
ir
geranyl diphosphate + ADP geranyl diphosphate is a very poor substrate Morus alba diphosphate + N6-geranyl-ADP
-
?
isopentenyl diphosphate + ADP isopentyl diphosphate is a very poor substrate Morus alba diphosphate + N6-isopentyl-ADP
-
?
additional information farnesyl diphosphate, geranylgeranyl diphosphate, IDP and UDP are no substrates for Morus alba AIPT Morus alba ?
-
?

Synonyms

Synonyms Comment Organism
adenylate isopentenyltransferase
-
Morus alba
AIPT
-
Morus alba

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0235
-
ADP
-
Morus alba
0.0345
-
dimethylallyl diphosphate
-
Morus alba

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7
-
Morus alba

Cofactor

Cofactor Comment Organism Structure
ADP
-
Morus alba
AMP
-
Morus alba
ATP
-
Morus alba