Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Morus alba |
Protein Variants | Comment | Organism |
---|---|---|
D49A | almost complete loss of activity compared to the wild type enzyme | Morus alba |
F120A | almost complete loss of activity compared to the wild type enzyme | Morus alba |
F157A | almost complete loss of activity compared to the wild type enzyme | Morus alba |
F93A | almost complete loss of activity compared to the wild type enzyme | Morus alba |
Q255A | almost complete loss of activity compared to the wild type enzyme | Morus alba |
W159A | almost complete loss of activity compared to the wild type enzyme | Morus alba |
Y153A | almost complete loss of activity compared to the wild type enzyme | Morus alba |
Y170A | retains 84% activity compared to the wild type enzyme | Morus alba |
Y217A | almost complete loss of activity compared to the wild type enzyme | Morus alba |
Y54A | almost complete loss of activity compared to the wild type enzyme | Morus alba |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0374 | - |
dimethylallyl diphosphate | - |
Morus alba | |
0.0682 | - |
ADP | - |
Morus alba |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
MgCl2 | dependent on presence of divalent cation, addition of 2 mM MnCl2 is 1.3 times more effective than that of 10 mM MgCl2 | Morus alba | |
MnCl2 | dependent on presence of divalent cation, addition of 2 mM MnCl2 is 1.3 times more effective than that of 10 mM MgCl2 | Morus alba |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35810 | - |
calculated from sequence of cDNA | Morus alba |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Morus alba | Q08ET4 | mulberry | - |
Purification (Comment) | Organism |
---|---|
Ni-chelate affinity column chromatography | Morus alba |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Morus alba | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxy-3-methyl-2-(E)-butenyl diphosphate + ADP | 4-hydroxy-3-methyl-2-(E)-butenyl diphosphate is a very poor substrate | Morus alba | diphosphate + N6-(4-hydroxy-3-methyl-2-(E)-butenyl)ADP | - |
? | |
4-hydroxy-3-methyl-2-(E)-butenyl diphosphate + ATP | 4-hydroxy-3-methyl-2-(E)-butenyl diphosphate is a very poor substrate | Morus alba | trans-zeatin riboside 5'-triphosphate + diphosphate | - |
? | |
dimethylallyl diphosphate + ADP | - |
Morus alba | diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate | - |
ir | |
dimethylallyl diphosphate + AMP | AMP is a poor substrate | Morus alba | diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate | - |
ir | |
dimethylallyl diphosphate + ATP | - |
Morus alba | diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate | - |
ir | |
dimethylallyl diphosphate + CDP | - |
Morus alba | ? | - |
ir | |
dimethylallyl diphosphate + dADP | - |
Morus alba | diphosphate + N6-(dimethylallyl)dADP | - |
ir | |
dimethylallyl diphosphate + dATP | - |
Morus alba | diphosphate + N6-(dimethylallyl)dATP | - |
ir | |
dimethylallyl diphosphate + GDP | GDP is a poor substrate | Morus alba | ? | - |
ir | |
geranyl diphosphate + ADP | geranyl diphosphate is a very poor substrate | Morus alba | diphosphate + N6-geranyl-ADP | - |
? | |
isopentenyl diphosphate + ADP | isopentyl diphosphate is a very poor substrate | Morus alba | diphosphate + N6-isopentyl-ADP | - |
? | |
additional information | farnesyl diphosphate, geranylgeranyl diphosphate, IDP and UDP are no substrates for Morus alba AIPT | Morus alba | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
adenylate isopentenyltransferase | - |
Morus alba |
AIPT | - |
Morus alba |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0235 | - |
ADP | - |
Morus alba | |
0.0345 | - |
dimethylallyl diphosphate | - |
Morus alba |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | - |
Morus alba |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Morus alba | |
AMP | - |
Morus alba | |
ATP | - |
Morus alba |